沉默/增强Stat1对胰腺癌细胞增殖和迁移的影响

目的研究沉默/增强Stat1对胰腺癌细胞增殖和迁移能力的影响。方法将胰腺癌细胞(Bx PC-3)分为:对照(control)组、Stat1组和si Stat1组,分别转染Stat1质粒和特异性干扰Stat1表达的siRNA;Western blot法检测Bx PC-3细胞中Stat1、VEGF、MMP-2和MMP-9蛋白的表达;MTT法绘制细胞增殖曲线;Transwell法检测Bx PC-3细胞侵袭能力。结果沉默Stat1表达后,Bx PC-3细胞增殖及迁移能力明显增加,且VEGF、MMP-2和MMP-9表达明显增加。而增强Stat1表达后,Bx PC-3细胞增殖及迁移能力明显降低(P0.05)。结论 Stat1可以抑制Bx PC-3细胞增殖及迁移能力。且VEGF、MMP-2和MMP-9可能在Stat1抑制肿瘤过程中起一定作用。

Effect of silenced/enhanced Stat1 geneon proliferation and invasion in pancreatic cancer cells

Objective To investigate the proliferation and invasion of human pancreatic cancer cells after Stat1 gene silenced or enhanced and to explore the possible mechanisms.Methods BxPC-3 cells were divided to three groups: Stat1 group transfected with specific Stat1 plasmid, Si Stat1 group transfected with specific Stat1 small interfering RNA (siRNA), and control group.Western blot was used to detect the expression of Stat1, VEGF, MMP-2 and MMP-9 in BxPC-3 cells.MTT method was used to detect the proliferation of BxPC-3 cells.Transwell was used to detect the invasion of BxPC-3 cells.Results The proliferation and invasion were significantly enhanced in pancreatic cancer cells after Stat1 gene silenced, and the expression of VEGF, MMP-2 and MMP-9 was increased.The proliferation and invasion were significantly decreased in pancreatic cancer cells after Stat1 gene enhanced.Conclusions Stat1 inhibits the proliferation and invasion of pancreatic cancer cells.VEGF,MMP-2 and MMP-9 possibly play a role in this process.