体外利用MGc80-3胃癌细胞诱导肿瘤杀伤细胞的实验研究

以健康人外周血单个核细胞作为肿瘤杀伤细胞的前体细胞，ＭＧｃ８０ ３胃癌细胞作为刺激细胞，在体外进行同种异体淋巴细胞和肿瘤细胞混合培养之后，单独加入重组白介素 ２和同时加入重组白介素 ２、抗ＣＤ３单克隆抗体，分别诱导出Ｌ 肿瘤杀伤细胞和ＣＤ３ 肿瘤杀伤细胞．用噻唑蓝比色法在体外检测其对ＭＧｃ８０ ３胃癌细胞的杀伤活性，并与淋巴因子 激活杀伤细胞、ＣＤ３ 肿瘤杀伤细胞相比较结果显示，ＣＤ３ 肿瘤杀伤细胞的杀伤活性最高．在倒置显微镜下及透射电镜下观察ＣＤ３ 肿瘤杀伤细胞对ＭＧｃ８０ ３胃癌细胞的杀伤过程，发现ＣＤ３ 肿瘤杀伤细胞首先接触、粘附靶细胞，以细胞坏死和细胞凋谢的方式杀伤肿瘤细胞

Tumor activated killer cells induced by gastric carcinoma cell line MGc80 3 in vitro

Tumor activated killer cells(TAK)were generated in human peripheral blood imononuclear cells by stimulating with human gastric carcinoma cell line MGc80 3 using allogenetic mixed lymphocyte tumor cell culture method. L TAK cells and CD3 TAK cells were induced by MGc80 3 cells with　rIL 2, and MGc80 3 cells with rIL 2,and anti CD3Ab,respectively.Testing in vitro cytotoxicity against MGc80 3 cells in MTT colorimetry CD3 TAK cells gave the highest levels of cytotoxity compared with LAK cells and CD3 AK cells.In vitro cytolysis process of MGc80 3 cells mediated by CD3 TAK cells were observed using inverted microscopy and transmission electron microscopy.The results showed that CD3 TAK cells attached the target and the target cells died by apoptosis and necrosis.