反流性食管炎、Barrett食管和食管腺癌中P38的表达特点

目的:观察反流性食管炎(RE)、Barrett食管(BE)、食管腺癌(EA)中食管上皮细胞的P38表达与细胞增殖凋亡的变化特点.方法:胃镜下取食管黏膜活检,免疫组化法和免疫印迹法测定RE组、BE组、EA组及正常对照组的P38和Ki-67的蛋白表达,TUNEL法测定细胞凋亡.结果:免疫组化法测定正常对照组、RE组、BE组、EA组的Ki-67表达率分别为10％、55％、60％、83.3％.TUNEL法测定正常对照组、RE组、BE组、EA组的细胞凋亡指数分别为(1.51±1.06)％、(8.12±1.55)％、(7.25±1.87)％、(3.63±1.70)％.免疫印迹法测定RE组、BE组的磷酸化P38蛋白吸光度比值分别为0.09±0.03、0.11±0.04,EA组和正常对照组无磷酸化P38蛋白表达.结论:与食管腺癌组相比,反流性食管炎、Barrett食管的细胞增殖率下降,细胞凋亡指数增加,伴随着磷酸化P38蛋白的表达增加,提示P38信号通路可能参与了反流性食管炎、Barrett食管发病过程中细胞增殖凋亡的调控.

Study on P38 expression in reflux esophagitis, Barrett esophagus and esophageal adenocarcinoma

Objective: To observe the P38 expression and cell proliferation-apoptosis in reflux esophagitis(RE),Barrett esophagus(BE)and esophageal adenocarcinoma(EA).Methods: The expression of P38 and Ki-67 were determined by immunohistochemistry technique and western blotting,and the cell apoptosises were determined by TUNEL in RE,BE,EA and the normal control,of which the esophageal mucous membrane biopsy were obtained by gastroscopy.Results: The Ki-67 expression ratio by immunohistochemistry were 10%,55%,60% and 83.3% in the normal control,RE,BE and EA,respectively.The cell apoptosis indexes by TUNEL were(1.51±1.06)%,(8.12±1.55)%,(7.25±1.87)% and(3.63±1.70)% in the normal control,RE,BE and EA,respectively.The phosphorylated P38 protein absorbance ratio by western blotting was 0.09±0.03 in RE and 0.11±0.04 in BE,with no phosphorylated P38 expression in EA and the normal control.Conclusion: Compared to the esophageal adenocarcinoma,there are the increased cell apoptosis and decreased cell proliferation accompanied by the up-regulation of phosphorylated P38 protein expression in reflux esophagitis and Barrett esophagus,which show P38 might be involved in the cell proliferation-apoptosis regulation in the pathogenesis of reflux esophagitis and Barrett esophagus.