低硒对小鼠心肌影响机制的研究

目的建立硒缺乏小鼠动物模型,观察低硒对小鼠心肌的影响机制。方法利用酵母配制低硒饲料,喂养昆明幼鼠,经过4个月的喂养,测定血清、肝脏、心肌细胞的硒含量,观察心肌超微结构的变化,测定血清心肌酶的变化。结果利用酵母配制的低硒饲料,硒含量为0.016mg/kg,符合低硒标准。昆明鼠用该饲料喂养4个月,心肌、肝脏、血清硒含量分别为0.187mg/kg、0.219mg/kg、0.241mg/kg,符合低硒诊断标准。观察低硒鼠心肌超微结构,可见心肌细胞线粒体肿胀,细胞核出现了异型性,血清心肌酶水平升高。结论经过低硒饲料饲养可建立低硒鼠动物模型。低硒主要引起昆明鼠心肌细胞线粒体的损伤。

Effects of Selenium-deficiency on mice myocardium

Objective To develop selenium-deficient Kunming mice by feeding with self-made selenium-deficient diet and observe the effects of selenium-deficiency on Kunming mice myocardium. Methods A selenium-deficient mouse food was prepared with local yeast.Young Kunming mice were fed with the selenium-deficent diet for over 4 months.Selenium concentrations in the blood,liver,and myocardial tissue of the mice fed with selenium-deficient diet were determinated.The myocardial enzymes in the treated mice were examined.The ultrastructures of the treated mice myocardial tissues were also observed. Results The selenium concentration in the mouse food prepared with local yeast was 0.016mg/kg and the standard of selenium-deficiency.The selenium levels in the serum,liver,and myocardial tissue of Balb/c mice fed with the selenium-deficient diet for 4 months were 0.241mg/kg,0.219mg/kg,and 0.187mg/kg.Swelling mitochondria and heteromorphic nucleus were observed in the selenium-deficient cardiomyocytes.The level of myocardial enzymes in the sera of the selenium-deficient mice increased. Conclusions A selenium-deficient mouse diet was successfully developed with local yeast,Selenium-deficient mouse can be prepared by feeding this diet.Selenium-deficiency mainly cause injuries in myocardial mitochondria of the Kunming mice.