| 和厚朴酚对人肝癌HepG2细胞增殖及NF-kB mRNA表达的影响 |
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| 引用本文： | 张玉碧,张辉,吕林华,贺小平. 和厚朴酚对人肝癌HepG2细胞增殖及NF-kB mRNA表达的影响[J]. 齐齐哈尔医学院学报, 2012, 33(12): 1562-1563 |
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| 作者姓名： | 张玉碧 张辉 吕林华 贺小平 |
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| 作者单位： | 张玉碧 (湖南省邵阳市中心医院肿瘤科,422000) ； 张辉 (湖南省邵阳市中心医院肿瘤科,422000) ； 吕林华 (中山大学附属第一医院,广东广州,422000) ； 贺小平 (湖南省邵阳市中心医院肿瘤科,422000) |
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| 基金项目： | 湖南省科技计划项目经费资助 |
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| 摘 要： | 目的探讨和厚朴酚对体外培养的人肝癌细胞HepG2的增殖抑制作用及其对NF-kB基因mRNA水平的影响。方法采用MTT法检测和厚朴酚对HepG2细胞增殖的影响。以RT-PCR方法研究和厚朴酚对NF-kB基因mRNA表达的影响。结果和厚朴酚使人HepG2细胞增殖率明显下降,且呈剂量依赖效应;NF-kB基因mRNA水平随和厚朴酚浓度升高而降低。结论和厚朴酚对人HepG2细胞增殖有抑制作用,并能下调NF-kB的表达,从而起到抗肿瘤的治疗作用。
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| 关 键 词： | 和厚朴酚 肝癌 细胞增殖抑制 NF-kB |
Effect and mechanism of Honokiol on proliferation of human liver cancer cell line HepG2 |
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| Affiliation: | ZHANG Yubi,et al.(Oncology Department,Central Hospital,Shaoyang,Hunan 422000,the P.R.China.) |
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| Abstract: | Objective To investigate the inhibitory effect of Honokiol on proliferation and the effect on the NF-kB gene mRNA level of human liver cancer cell line HepG2 in vitro.Methods Cell proliferation was evaluated by Methyl thiazolyl tetrazolium(MTT) assay.NF-kB gene mRNA level was examined by RT-PCR method.Results Honokiol reduced the ratio of the proliferation of the HepG2,and was dose-dependent;the level of mRNA of NF-kB gene increased with the concentration of Honokiol decreased.Conclusions Honokiol play the role of anti-tumor therapy by inhibiting the prefloration of HepG2 cell and decreasing the level of mRNA in NF-kBgene. |
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| Keywords: | Honokiol Liver cancer Eytostasis NF-kB |
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