乳腺癌抑癌基因蛋白P16的检测及其临床意义

目的]探讨抑癌基因蛋白P16与乳腺癌发生和发展的关系。方法]采用免疫组织化学SP法检测65例乳腺癌（淋巴结转移21例、非淋巴结转移44例）20例乳腺增生病、20例乳腺纤维腺瘤组织P16蛋白的表达情况，组间率的比较采用卡方检验，并分析其表达与患者临床及病变情况的关系。结果]P16蛋白在乳腺癌、乳腺增生、乳腺纤维腺瘤组的阳性表达率分别为49％、50％及85％，其表达在乳腺癌、乳腺增生与乳腺纤维腺瘤之间进行比较差异显著（P〈0．05）；P16蛋白在淋巴结转移组和非淋巴结转移组中的表达分别为69％、36％，差异性显著（P〈0．05）；其与病理组织学类型、肿瘤大小、5年生存率情况比较无明显相关性。结论]P16蛋白表达异常在乳腺癌的发生发展中起着一定的作用。

Significance of detecting P16 protein expression in diagnosing of breast cancer

Objective] To study the significance of P16 expression of breast cancer.Method] Immunohistochemical method was used to detect the expression of P16 in 65 cases of breast cancer,including 21 cases with and 44 cases without lymphnodo metastasis,20 cases of breast atypical hyperplasia and 20 cases of breast fibroadenoma.Results] The positive expression rates of P16 protein in breast cancer,breast atypical hyperplasia and breast fibroadenoma groups were 49%,50% and 85% respectively.There was significant difference between breast cancer or breast atypical hyperplasia and breast fibroadenoma(P>0.05).The positve rates p16 expresstion in cases with and without lymph nodometastasis were 69% and 36% respectively(P<0.05).Conclusion] Detection of p16 may be useful in diagnosis of breast cancer.Protein P16 play an important role in the breast carcinogenesis.