植物血凝素激活的LAK细胞激活培养条件与其生物学特性关系的研究

将ＰＨＡ和ｒＩＬ ２合理配伍应用于ＬＡＫ细胞的激活培养中，研究了ＰＨＡ ＬＡＫ细胞的增殖、细胞毒活性及表型。结果表明：培养条件中加入适量ＰＨＡ可促进ＰＨＡ ＬＡＫ的增殖（Ｐ＜０．０５），不影响其细胞毒活性。ＰＨＡ／ｒＩＬ ２共同预刺激组于培养第２ｄ细胞数明显下降（３０％～４０％），但其到达增殖高峰的时间、峰值及扩增倍数与同一培养条件下的ＰＨＡ单独预刺激组相比，差别无显著性（均为Ｐ＞０．０５）；但前者对Ｋ５６２和Ｒａｊｉ的杀伤高峰值大于后者（Ｐ＜０．０１）。各组ＰＨＡ ＬＡＫ中有０．６０～０．７７的细胞为ＣＤ３＋Ｔ细胞，但ＰＨＡ ＬＡＫ／ｒＩＬ ２共同预刺激者的ＣＤ３＋、ＣＤ８＋细胞所占比率较ＰＨＡ单独预刺激者为高（Ｐ＜０．０５）。

Study on the Relationship between the Preming and(or) Cultivating Condition of Human PHA LAK Cells and their Biological Charateristics

Some biological charateristics(proliferation response, cytotoxicity,phenotype)of PHA-LAKcells under different preming and cultivating conditions were studied. The proliferation responses showed that the number of PHA-LAKcells after co priming for 48 h with PHA/rIL-2 declined dramatically during the first two days, and reached the peak value of proliferation on the same day(day 6 after priming)as did those PHA-LAKcells primed with PHAalone under the same cultivating condition, and the differences in the peak value and multiplication fold on every same day between the PHApreming alone and the PHA-LAKco priming group were statistically insignificant(P>0.05). The cytotoxicity results showed that the peak time of cytotoxicity of PHA-LAKcells co primed with PHA/rIL-2 against K₅₆₂ and Raji cell reached on day 6 after co priming, two days earlier than that of PHA-LAKcells premied with PHAalone and the peak value of the former against Raji cells was higher than that of the latter(P<0.01). The phenotype analysis showed that about 0.60～0.77 of PHA-LAKcells were CD3⁺ cells under different priming and cultivating condition, and CD3⁺,CD8⁺ cells of PHA-LAKprimed with PHA/rIL-2 were more than those of PHA-LAKprimed with PHAalone(P<0.05).