人类鼻咽癌端粒酶活性的研究及其临床意义

目的 探讨端粒酶在鼻咽癌中的表达及其临床意义。方法 采用ＴＲＡＲ－银染法对４２例鼻咽部低分化鳞状细胞癌、１０例鼻咽部正常粘膜和８例鼻咽纤维血管瘤进行端粒酶活性检测及血清ＶＣＡ／ＩｇＡ检测，并分析其与临床病理的关系。结果 鼻咽部正常粘膜和鼻咽癌端粒酶阳性率分别为２０％和８８．１％，８例鼻咽纤维血管瘤均未检测到端粒酶活性。鼻咽癌伴颈部淋巴结转移者端粒酶阳性率高于无颈部淋巴结转移者（Ｐ＝０．０３５），临

The study on the role of telomerase activity in human nasopharyngeal carcinoma]

OBJECTIVE: To elucidate the role of telomerase activity in nasopharyngeal carcinoma (NPC). METHODS: Telomerase activity was detected using the telomere repeat amplification protocol(TRAP) combined with silver staining in 42 low-differentiated squamous carcinoma, 10 normal nasopharyngeal mucosae and 8 nasopharyngeal fibrohemangioma. RESULTS: Telomerase activity was detectable in 20% normal nasopharyngeal mucosae and 88.1% in NPC. The activity of telomerase was not detectable in 8 nasopharyngeal fibrohemangioma tissues. Telomerase activity was present more frequently in NPC biopsies with positive lymph node metastasis than in those without lymph node metastasis (P = 0.035). Furthermore, telomerase activity was observed more frequently in advanced NPC than in early stage (P = 0.048). However, there was no evidence to show the relationship between telomerase activity and other clinical features, such as age, gender and distant metastasis (P > 0.05). Serum EBV marker(VCA-IgA) was observed more frequently in NPC than in normal nasopharyngeal mucosae patients(P < 0.001). CONCLUSION: Telomerase activity may play a critical role in the tumorigenesis of NPC. Detection of telomerase activity combined with serological EBV markers may contribute to the screening and early diagnosis of NPC.