rhBMP-2及rhbFGF对骨髓间充质干细胞增殖的长期效应的实验研究

目的：检测rhBMP-2和rhbFGE、单独、联合及序贯作用对兔骨髓间充质干细胞增殖的长期效应。方法：在细胞培养技术下，采用MTT法检测单独、联合及序贯施加rhBMP-2和rhbFGF后，兔骨髓间充质干细胞在第1，3，5，7及10天增殖的状况，并进行统计学分析。结果：rhBMP-2和rhbFGF均能长期显著地促进兔骨髓间充质干细胞的增殖，并呈剂量依赖性。两种生长因子联合作用的效应高于单独或序贯作用的效应。结论：不同生长因子的不同应用方式对骨髓间充质干细胞增殖的长期效应不同。

The Long-term Effects of rhBMP-2 and rhbFGF on Proliferation of Bone Mesenchymal Stem Cells

Objective:To evaluate the long-term effects of rhBMP-2 and rhbFGF on proliferation of rabbit bone mesenchymal stem cells by using them alone, associatedly and sequentially.Methods:Cell culture technique and MTT colorimetric assay were used to evaluate the proliferation of rabbit bone mesenchymal stem cells on the 1st, 3rd, 5th, 7th, and 10th day by adding rhBMP-2 and rhbFGF on different ways.Results:Statistical analysis showed that both rhBMP-2 and rhbFGF could enhance the proliferation of rabbit bone mesenchymal stem cells significantly in long term, and also in a dose-dependent manner.The effects of the two growth factors were significantly higher by using them associatedly than by using them alone or sequentially.Conclusions:In tissue engineering work, we can get a great quantity seed cells in a short time by using different growth factors associatedly.