| genistein多途径抑制乳腺癌细胞生长和侵袭 |
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| 引用本文： | 邵志敏,吴炅,沈镇宙.genistein多途径抑制乳腺癌细胞生长和侵袭[J].中华肿瘤杂志,2000,23(5):362-365. |
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| 作者姓名： | 邵志敏 吴炅 沈镇宙 |
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| 作者单位： | 上海医科大学肿瘤医院胸外科分子生物实验室 |
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| 摘 要： | 目的 研究ｇｅｎｉｓｔｅｉｎ在乳腺癌细胞生长和侵袭中的作用。方法 应用体外细胞培养和体内乳腺癌细胞移植裸鼠模型以及酶谱分析、Ｎｏｒｔｈｅｎ和Ｗｅｓｔｅｒｎ ｂｌｏｔ方法。结果 在体外实验中，ｇｅｎｉｓｔｅｉｎ不仅具有抑制乳腺癌细胞生长的作用，还具有抑制乳腺癌细胞侵袭的功能，这种抑制功能是伴ｇｅｎｉｓｔｅｉｎ能明显抑制蛋白金属酶ＭＭＰ－９的表达和增加组织金属蛋白酶抑制剂ＴＩＭＰ１的表达。ｇｅｎｉｓｔ
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| 关 键 词： | 乳腺肿瘤 肿瘤侵袭 细胞凋亡 |
Genistein exerts multiple suppressive effects on human breast carcinoma cells |
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| Z Shao,J Wu,Z Shen.Genistein exerts multiple suppressive effects on human breast carcinoma cells[J].Chinese Journal of Oncology,2000,23(5):362-365. |
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| Authors: | Z Shao J Wu Z Shen |
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| Institution: | Department of Surgery, Molecular Biology Laboratory, Cancer Hospital/Cancer Institute, Shanghai Medical University, Shanghai 200032, China. |
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| Abstract: | OBJECTIVE: To study the suppressive effects of genistein on progression of breast carcinoma. METHODS: In vitro cell culture, animal studies, zymography, Northern and Western blot were used in this study. RESULTS: Genistein inhibited in vitro invasion of MCF-7 and MDA-MB-231 cells. This inhibition was associated with down-regulation of MMP-9 and up-regulation of TIMP-1. These effects were also demonstrated in vivo. Genistein inhibited growth of the breast cancer cells transplanted in nude mice. The growth inhibitory effect was associated with increased tumor cell apoptosis, and up-regulated p21WAF1/CIP1 expression. Furthermore, genistein inhibited angiogenesis as shown by decreased vessel density and decreased production and release of VEGF and TGF-beta 1. CONCLUSION: Genistein exerts multiple suppressive effects on breast carcinoma. It may help halt its progression. |
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