多烯紫杉醇靶向放疗增敏对结肠癌细胞凋亡及相关蛋白表达的影响

目的：探讨免疫脂质化的多烯紫杉醇在LoVo细胞放射增敏过程中， 对其凋亡及凋亡相关蛋白表达的影响。方法：制备偶联癌胚抗原（CEA）抗体的多烯紫杉醇脂质体，作用于LoVo细胞后再经2Gy照射，然后再进行相关检测：DNA末端原位标记染色法(TUNEL) 计算凋亡指数(AI);检测p53和Bax和Bcl-2，Fas，FasL及survivin 的表达。结果：多烯紫杉醇免疫脂质体联合放射组（A组）LoVo的AI为(43.6±5.2)% ，均显著高于多烯紫杉醇脂质体联合放射组（B组） (17.7±3.9)%及单纯放射组（C组） (16.8±3.5)% （P< 0. 01）。而B,C组间差异无统计学意义（P>0. 05）。A组的Bax,Fas和FasL的表达显著高于B组;而A组的survivin的表达显著低于B组（P<0.01）,但两组对p53和Bcl-2表达影响均无明显差异。结论：多烯紫杉醇经多种通路调节细胞凋亡，并能增加肿瘤细胞的放疗敏感性。

The effect of immunoliposomal docetaxel in combination with radiation on the expression of apoptosis-related proteins in LoVo cell line

Abstract:Objective： To investigate immunoliposomal docetaxel in combination with radiation on the expression of apoptosis-related proteins in LoVo cell line.Methods ：Immunoliposomal docetaxel was prepared by coupled CEA antibody. LoVo cells were treated with immunoliposomal docetaxel and irradiation (group A), or with liposomal docetaxel and irradiation (group B), or irradiation alone (group C). The apoptosis index (AI) was evaluated by TUNEL method. The expressions of proteins p53, Bcl-2 and Bax were detected by flow cytometry. Fas、FasL and survivin proteins were detected by immunohistochemical staining and were quantified using semi-quantitative analysis by image analysis system. Results：AI in group A (43.6%) was Significantly higher than that in group B (17.7 %) and group C (16.8%)(P<0.01).but no significant difference between group B and group C (P>0.05). Bax, Fas and FasL expression in group A were significantly higher than those in group B, but survivin expression in group A was lower than thatin group B (P<0.01).Conclusions：Different pathways are involved in promotion of apoptosis in cancer cells that undergo docetaxel treatment, and decetaxel can enhance LoVo cells radiosensitization.