慢性粒细胞白血病患者树突状细胞抗原加工递呈和迁移功能观察

目的:研究细胞因子体外联合诱导培养的慢性粒细胞白血病(CML)患者树突状细胞(DCs)的表型和抗原递呈、分泌和迁移功能的变化.方法:分离CML患者(CML组)和健康志愿者(正常组)外周血单个核细胞(PMNCs),经培养获取贴壁细胞,将CML组和正常组的贴壁细胞及K562细胞用细胞因子rhGM-CSF、rhIL-4、TNF-α体外诱导10 d后,流式细胞仪检测细胞的免疫表型;培养5 d后,用ELISA检测细胞的内吞功能;应用混合淋巴细胞培养分析3组DCs对静息T细胞的免疫刺激活性.结果:正常组、CML组、K562组DCs的特异性标志CD83和共刺激分子标记CD80和CD86以及CD11和HLA-DR的表达差异无统计学意义(P＞0.05);DCs的抗原吞噬、迁移能力和对T细胞的免疫刺激活性,CML组和K562组之间差异无统计学意义(P＞0.05),CML组和正常组间差异有统计学意义(P＜0.05).结论:CML患者DCs的免疫表型和正常人相似,但其抗原捕获、递呈和迁移功能有缺陷.

Investigation on antigen processing and presentation, migration ability of dendritic cells in vitro from chronic myeloid leukemia patients

Aim: To study the antigen processing and presentation, secretion, and migration ability of dendritic cells(DCs) from chronic myeloid leukemia (CML) patients. Methods:The differentiation of DCs generated from CD34 progenitor cells was mediated by cytokines like granulocyte macrophage colony-stimulating factor, tumor necrosis factor, interleukin-4. Maturation status of CML DCs and normal DCs were evaluated by the surface marker using flow cytometry. Ability of DCs to stimulate T cells and capture antigen were detected using a liquid-scintillation counter and ELISA.Results: Phenotypic analysis of CML and normal DCs showed a similar surface expression of maturation makers of CD80, CD86, CD83, CD11, and HLA-DR (P>0.05). There are statistical differences in the capacity of T cells activation, endocytosis or phagocytosis and transmigration between DCs derived from CML patients and healthy individuals.Conclusion: DCs derived from both healthy subjects and patients with CML differentiated and matured cultured in vitro in a similar way. However, DCs generated from CML patients have a reduced capacity to capture,process and present antigen and migration when compared with DCs from healthy controls.