The effect of acteoside on intracellular Ca2+ mobilization and phospholipase C activity in RBL-2H3 cells stimulated by melittin

This study was performed to investigate the effects of acteoside on various cellular functions such as, intracellular Ca²⁺ mobilization, phospholipase C activity, and exocytosis induced by melittin. Melittin (0.1–1 μM) dose-dependently increased intracellular Ca²⁺ mobilization in the presence of extracellular Ca²⁺, but was not affected by 1 μM U73122, a specific PLC inhibitor. In the absence of extracellular Ca²⁺, melittin (1 μM) did not induce a change in intracellular Ca²⁺ mobilization, which suggests that melittin-induced intracellular Ca²⁺ mobilization may be dependent on the influx of extracellular Ca²⁺ rather than on the release of intracellular Ca²⁺ storage. Acteoside (10 μM) significantly inhibited 1 μM melittin-induced Ca²⁺ mobilization by 33 %. In [³H]inositol-labeled cells, 1 μM melittin did not increase inositol phosphate formation, but more than 5 μM melittin significantly increased inositol phosphate formation, which was significantly inhibited by acteoside. Melittin (1 μM) significantly increased histamine release from RBL 2H3 cells in the presence or absence of extracellular Ca²⁺. Acteoside significantly inhibited 1-μM-melittin-induced histamine release by 74 % in the presence of extracellular Ca²⁺ and by 71 % in the absence of extracellular Ca²⁺. These data suggest that the inhibitory effect of acteoside on 1 μM-melittin-induced histamine release may be related to blockage of the calcium-independent pathway. Taken together, these data suggest that melittin has an influence on cellular functions such as intracellular Ca²⁺ mobilization, the PLC pathway, and exocytosis via various independent signalling pathways in RBL-2H3 cells, and was significantly inhibited by acteoside.