| 清开灵注射液中杂蛋白的检测方法研究 |
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| 引用本文： | 王新彤,唐思园,卢建秋,史新元.清开灵注射液中杂蛋白的检测方法研究[J].世界科学技术-中医药现代化,2012,14(3):1663-1666. |
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| 作者姓名： | 王新彤 唐思园 卢建秋 史新元 |
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| 作者单位： | 北京中医药大学中药学院 北京 100102 |
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| 基金项目： | 北京市教育委员会共建项目：中药注射剂安全性研究技术平台建设，负责人：卢建秋。 |
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| 摘 要： | 目的:建立清开灵注射液中大分子杂蛋白的检测方法。方法:以三氯乙酸沉淀结合双缩脲法初步测定清开灵注射液中杂蛋白的浓度,再用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)检测杂蛋白的分子量。结果:清开灵注射液成品中杂蛋白的分子量在3～7 KD之间。结论:本方法可用于检测清开灵注射液中的大分子杂蛋白。
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| 关 键 词： | 清开灵注射液 杂蛋白 SDS-PAGE |
| 收稿时间： | 2011/7/28 0:00:00 |
| 修稿时间： | 8/5/2011 12:00:00 AM |
Study on Protein Detection Method in Qingkailing Injection |
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| Wang Xintong,Tang Siyuan,Lu Jianqiu and Shi Xinyuan.Study on Protein Detection Method in Qingkailing Injection[J].World Science and Technology-Modernization of Traditional Chinese Medicine,2012,14(3):1663-1666. |
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| Authors: | Wang Xintong Tang Siyuan Lu Jianqiu and Shi Xinyuan |
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| Institution: | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China |
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| Abstract: | This study was aimed to establish a method for determining protein content in Qingkailing Injection. Biuret colorimetry was used to determine the protein which had been precipitated by trichloroacetic acid. SDSpolyacrylamide gel electrophoresis (SDS-PAGE) was used to determine the molecular weight of the protein. The results showed that molecular weight of protein in Qingkailing Injection is between 3 KD to 7 KD. It was concluded that SDS-PAGE can be used to determine the protein in Qingkailing Injection. This method is simple and rapid. |
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| Keywords: | Qingkailing Injection protein SDS-PAGE |
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