| 高效液相色谱-质谱联用法研究巫山淫羊藿黄酮类成分 |
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| 引用本文： | 谭鹏,徐娟,仲玲利,雷海民,杨蕾,李飞. 高效液相色谱-质谱联用法研究巫山淫羊藿黄酮类成分[J]. 世界科学技术-中医药现代化, 2012, 14(3): 1667-1671 |
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| 作者姓名： | 谭鹏 徐娟 仲玲利 雷海民 杨蕾 李飞 |
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| 作者单位： | 北京中医药大学中药学院 北京 100102 |
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| 基金项目： | 国家自然科学基金项目（30873436）：巫山淫羊藿油炙增效机理的研究，负责人：李飞。 |
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| 摘 要： | 目的:采用高效液相色谱-质谱联用法(HPLC-MSn)研究巫山淫羊藿中的黄酮类成分。方法:70%乙醇提取,大孔树脂柱纯化得到总黄酮;运用HPLC-MSn联用技术得到各化合物的总离子流图和多级质谱图。结果:从巫山淫羊藿药效部位中鉴定出12个化合物,其中有3对化合物互为同分异构体。结论:HPLC-MSn法可用于巫山淫羊藿中黄酮类成分的结构鉴定,为建立快速、准确的质量评价方法和分析炮制前后成分变化提供了参考。
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| 关 键 词： | 巫山淫羊藿 黄酮 HPLC-MSn |
| 收稿时间： | 2011-07-15 |
| 修稿时间： | 2011-08-03 |
HPLC-MSn Analysis of Flavonoids in Epimedium wushanense T. S. Ying |
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| Tan Peng,Xu Juan,Zhong Lingli,Lei Haimin,Yang Lei and Li Fei. HPLC-MSn Analysis of Flavonoids in Epimedium wushanense T. S. Ying[J]. World Science and Technology—Modernization of Traditional Chinese Medicine and Materia Medica, 2012, 14(3): 1667-1671 |
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| Authors: | Tan Peng Xu Juan Zhong Lingli Lei Haimin Yang Lei Li Fei |
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| Affiliation: | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China |
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| Abstract: | This study was aimed to investigate the flavonoids of Epimedium wushanense T. S. Ying using HPLC-MSn. The sample was extracted with 70% ethanol and purified by macroporous resin. The structures were detected by HPLC-MSn experiments. The results showed that twelve compounds were identified from the effective part of Epimedium wushanense T. S. Ying. There are three pairs of isomeric compounds. It was concluded that this method can be used for rapid and accurate identification of flavonoids. It can also be used as reference in the analysis of chemical constituent changes before and after drug processing. |
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| Keywords: | Epimedium wushanense T. S. Ying flavonoid HPLC-MSn |
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