改良小鼠酒精性肝损伤模型的建立

目的探索建立简便和稳定的酒精性肝病（ALD）的动物模型。方法利用简易胃造漏的方法,将实验动物分为四组,A组为正常小鼠（n=12）;B组为胃造瘘后给予生理盐水（n=12）;C组为胃造瘘后给予酒精（18g·kg-1·d-1）（n=22）;D组小鼠给予自制的ZQ液（n=22）。实验动态观察12周,分别在第4周、6周、8周、12周测四组小鼠肝重、体重值并检测肝脏ALT和AST水平,同时收集肝脏标本经HE染色后光镜观察肝组织的结构变化。结果 C组模型与A组和B组比较也出现不同程度的肝脏损害,但损害的程度较D组轻。D组小鼠在4周开始出现了肝细胞脂肪变性,8周开始出现酒精性肝炎和轻度的肝纤维化,12周时出现肝脏纤维化的表现。结论简易胃造瘘法配合ZQ液可以建立简便和稳定的酒精性肝损伤模型。

Modelling the chronic alcoholic liver disease in mouse

Objective To establish a flexible and stable alcoholic liver disease （ALD） model of mice.Methods Animals were randomly divided into four groups.Group A were normal mice（n=12） ;Group B were given normal saline after gastrostomy（n=12） ;Group C were given alcohol after gastrostomy （18 g·kg-1·day-1） （n=22） ;Group D were given homemade ZQ fluid（n=22）.The liver ALT and AST levels were dynamically monitored for 12 weeks.The mouse from every group were measured liver weight,body weight and detected levels of ALT and AST at the 4,6,8,12 weeks respectively when the liver were collected at the same time.The pathologic change of hepatic tissue in both groups was observed respectively in light microscope after HE staining.Results Compare to group A and group B,live of group C was damaged obviously,but lighter than that of group D.Four weeks later,group D beginning to emerge fatty degeneration.At 8 weeks group D began to appear in alcoholic hepatitis and mild liver fibrosis,and the liver become fibrosis at 12 weeks.While no pathologic change was found in the control group.Conclusions Simple gastric fistula with ZQ fluid transfusion can establish the flexible and stable model of alcoholic liver injury.