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E花结试验检测环境化学接触人群免疫效应的探讨
引用本文:赵美英,李晓燕,应晨江,朱贤述. E花结试验检测环境化学接触人群免疫效应的探讨[J]. 中国公共卫生学报, 1992, 0(1)
作者姓名:赵美英  李晓燕  应晨江  朱贤述
作者单位:同济医科大学,同济医科大学,同济医科大学,同济医科大学 武汉 430030,武汉 430030,武汉 430030,武汉 430030
摘    要:E花结试验受多种因素的影响。本文将洗涤绵羊红细胞(SRBC)的离心速度(2000r/min)减慢1.0倍,E花结形成细胞(ERFC)的活性(Ea)及总数(Et)分别提高3.5及2.6倍。在采集血样前12h限制脂肪摄入量。结果Ea及Et分别提高4.3和3.0倍。研究证明:控制样品条件及洗涤SRBC的离心转速,可提高本方法的可靠性。

关 键 词:E花结试验  影响因素  氯乙烯

Erythocytes Rosette Forming Test in Detection of Immunologic Effects of Environment Chemical Pollution on Population
Zhan Meiying,et al.. Erythocytes Rosette Forming Test in Detection of Immunologic Effects of Environment Chemical Pollution on Population[J]. , 1992, 0(1)
Authors:Zhan Meiying  et al.
Affiliation:Wuhan 430030
Abstract:There are several factors that can influence the results of erythrocyte rosette forming test. The rates of the erythrocyte rosette activity (Ea) and the total Erythrocyte rosette activity(Et) are increased by 3.5 and 2.6 times separately while the speed of centrifuge for washing sheep red blood cells (SRBC) is decreased to 1000 r/min (half of the previous speed). In the meantime, the Ea and Et will be three times higher if the fat intake of subjects is limited 12 hours before serum sample collection. The study shows that the reliability of erythrocyte rosette forming test can be improved by controling sample quality and decreasing the centrifugal speed.
Keywords:Erythrocyte rosette forming test Influence factors Vinyl Chloride
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