卡托普利对持续心肌缺血模型动物心肌细胞凋亡及凋亡相关基因的影响

目的:研究卡托普利对持续心肌缺血心肌细胞凋亡以及对凋亡相关基因的影响。方法:采用大鼠动物模型,用原位标记与半定量分析检测心肌细胞凋亡程度;以S-P免疫组化分别检测Fas基因蛋白。结果:持续心肌缺血组与假手术组相比,心肌细胞凋亡程度明显增加,Fas-L蛋白、Bcl-2蛋白的表达都有明显增高,差异具极显著性(P<0.01);卡托普利组与持续心肌缺血组比较,心肌细胞凋亡程度明显降低,差异具极显著性(P<0.01);Bcl-2蛋白表达增高,差异有显著性(P<0.05)、Fas-L蛋白表达降低,差异有显著性(P<0.05)。结论:卡托普利能够减轻持续心肌缺血过程中心肌细胞凋亡程度。

Effects of captopril on apoptosis of myocardial cells and gene due to apoptosis during continuous ischemia

OBJECTIVE To study the effects of captopril on apoptosis of myocardial cells and gene due to apoptosis during continuous ischemia.METHODS The degrees of myocardial apoptosis were identified by in situ end labeling and semiquantitive anlysis,Fas-gene protein was detected by S-P immunochemistry.RESULTS Comparing continuous ischemia group with sham operation group,the degrees of myocardial apoptosis improved obviously,the Fas-L proteins and Bcl-2 proteins expression was significantly raised,there was significantly difference(P<0.01);Comparing given group with continuous ischemia group,the degree of myocardial cells apoptosis was obviously reduced,there was significantly difference(P<0.01);Bcl-2 proteins expression was enhanced,there was difference(P<0.05);Fas-L proteins expression was lowed,there was difference(P<0.05).CONCLUSION Captopril could reduce the degrees of myocardial cells apoptosis during continuous ischemia.