银杏叶提取物对大鼠视网膜神经节细胞的保护作用

目的研究急性高眼压缺血再灌注模型中银杏叶提取物对视网膜神经节细胞的保护作用。方法实验用SD大鼠30只分为3组,分别为阴性对照组、阳性对照组、用药组,每组各10只。大鼠右眼为损伤眼,分别行前房穿刺灌注形成110mmHg的眼压维持60min,左眼未做损伤,作为正常对照。阴性对照组于损伤后2h及此后每日腹腔分别灌胃生理盐水5mL/kg。按这种给药方法,阳性对照组给予1%灯盏细辛5mL/kg,用药组给予1%银杏叶提取物5mL/kg,实验持续时间28d。动物安死术前5d用3%荧光金双上丘注射,逆行标记视网膜神经节细胞。做视网膜定向铺片,距离视乳头中心上下左右各2mm拍摄照片。利用彩色颗粒分析软件做节细胞计数,计算各组节细胞的存活率并做统计分析。结果阴性对照组、阳性对照组、用药组节细胞存活率分别为61.46%、72.73%、76.20%。用药组与阴性对照组的节细胞存活率存在显著性差异(P<0.05),用药组与阳性对照组无显著性差异(P>0.05),阳性对照组与阴性对照组之间存在显著性差异(P<0.05)。结论在急性高眼压缺血再灌注模型中,银杏叶提取物能有效地保护视网膜节细胞,与阳性对照药灯盏细辛比较,保护节细胞的效果两者接近。

Neuroprotective Effect of Extact of Ginko Biloba on the Retinal Ganglion Cells

Objective To investigate whether the extact of ginko biloba (EGb761) can protect the retinal ganglion cells (RGC) after acute high intraocular pressure induced retinal ischemia-reperfusion injury. Methods Thirty Sprague-Dawley rats were randomly divided into three groups. The negative control group, positive control group, treatment group were given normal saline 5 mL/kg, 1% erigeron breviscapus (vant) hand-mazz (EBHM) 5 mL/kg, 1% EGb761 5 mL/kg, respectively 2 hour after injury and every day after operation. Retinal ganglion cells were retrograde labeled by injection of 3% fluorogold (FG) into both side of superior colliculus 5 days before the death of the rats. Results The average survival rate of retinal ganglion cells were 61.46%, 72.73% and 76.20% in negative control group, positive control group and treatment group respectively. Conclusion EGb761 has neuroprotective effect on retinal ganglion cells in acute high intraocular pressure induced retinal ischemia-reperfusion injury.