急性坏死性胰腺炎时NO变化及三七总甙的治疗作用

目的:探讨急性坏死性胰腺炎(ANP)大鼠体内一氧化氮(NO)在疾病发展过程中的变化规律,中药三七总甙对ANP的治疗作用及对NO的影响.方法:将120只SD大鼠随机分为假手术组、对照组及实验组,用5%牛磺胆酸钠制成ANP模型,实验组用三七总甙治疗,对照组不用药物治疗,分别于术后2 h、4 h、6 h、8 h、12 h、24 h测定血清及胰腺的NO含量,并比较生存率.结果:实验组和对照组术后2 h NO水平显著增高,8 h后开始降低,24 h仍高于正常水平.但实验组明显低于对照组,实验组的生存率亦明显高于对照组.1周生存率假手术组、ANP组、ANP 中药组分别为100%、0%、70%.结论:ANP时NO有显著变化,三七总甙对其有抑制作用,对ANP有显著的治疗作用.

Changes of Nitric Oxide in Acute Necrotizing Pancreatitis and Therapeutic Action of Sanqizongdai(三七总甙)

Objective To study the changes of nitric oxide(NO)levels in plasma and the pancreas of acute necrotizing pancreatitis(ANP)rats,and the therapeutic result of Sanqizongdai Methods ANP was induced by retro-grade injection of 5% sodium taurocholate into the biliary-pancreatic duct.One hundred and 20 rats were randomly assigned to three groups with different treatments:sham-operation(n=40),ANP treated with normal saline(NS)(n=40),and ANP treated with Sanqizongdai(n=40).Serum amylase was determined at 6h,12h and 24h,the nitric oxide levels in plasma and in the pancreas were examined at 2h,4h,6h,8h,12h,24h.The one-week mortalities in the three groups were recorded.Results The levels of NO increased significantly in ANP rats.Sanqizongdai could reduce the levels on nitric oxide in plasma and the pancreas in rats with ANP.The survival rate of rats in Sanqizongdai was significantly higher than that of rats in NS group. Conclusion Sanqizongdai showed beneficial effects in the treatment of ANP through decreasing the nitric oxide levels in plasma and the pancreas.