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| 用RP-HPLC法同时测定芩梅颗粒中黄芩苷与阿魏酸的含量 | |
| 引用本文： | 房俨,顾清,杨帝顺,宋洪杰,刘继勇.用RP-HPLC法同时测定芩梅颗粒中黄芩苷与阿魏酸的含量[J].药学服务与研究,2012,12(5):370-373. |
| 作者姓名： | 房俨 顾清 杨帝顺 宋洪杰 刘继勇 |
| 作者单位： | 1. 第二军医大学长海医院药学部,上海200433;江苏省徐州市钟吾卫校,徐州221400 2. 上海市闸北区中心医院药剂科,上海,200070 3. 同济大学附属东方医院药学部,上海,200120 4. 第二军医大学长海医院药学部,上海,200433 |
| 基金项目： | 上海市科学技术委员会中药现代化专项基金资助项目(No.11DZ1971400);上海市卫生局中医药科研基金资助项目(No.2010Y004B) |
| 摘 要： | 目的:建立同时测定芩梅颗粒中黄芩苷和阿魏酸含量的RP-HPLC法.方珐:色谱柱:Platisial ODS C18柱(250 mm×4.6 mm,5 μm)；流动相:乙腈-0.085％磷酸水溶液(24∶76),流速:1.0 ml/min,检测波长:321 nm.结果:黄芩苷在1.09～ 109.12 μg/ml范围内线性良好(r=0.9998)；阿魏酸在1.09～ 108.64 μg/ml范围内线性良好(r=0.9999);日内和日间精密度、稳定性、重复性良好.黄芩苷低、中、高浓度的加样回收率分别为(99.69±1.24)％、(96.90±0.28)％和(95.06±0.44)％(n=3),阿魏酸低、中、高浓度的加样回收率分别为(95.90±1.13)％、(96.70±0.30)％和(102.52±1.18)％(n=3).3批芩梅颗粒样品中黄芩苷的含量为(5.85±0.016) mg/g(n=3),阿魏酸的含量为(90±1.8) μg/g(n=3).结论:该方法准确、灵敏,专属性强,可用于芩梅颗粒的含量测定. |
| 关 键 词： | 芩梅颗粒 黄芩苷 阿魏酸 含量测定 色谱法 高效液相 |
Simultaneous determination of contents of baicalin and ferulic acid in Qinmei granules by RP-HPLC method |
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| FANG Yan , GU Qing , YANG DiShun , SONG HongJie , LIU JiYong.Simultaneous determination of contents of baicalin and ferulic acid in Qinmei granules by RP-HPLC method[J].Pharmaceutical Care and Research,2012,12(5):370-373. | |
| Authors: | FANG Yan GU Qing YANG DiShun SONG HongJie LIU JiYong |
| Institution: | 1*(1.Department of Pharmacy,Changhai Hospital,Second Military Medical University,Shanghai 200433,China;2.Xuzhou Zhongwu Medical School of Jiangsu Province,Xuzhou 221400,China;3.Department of Pharmacy,Central Hospital of Zhabei District,Shanghai 200070,China;4.Department of Pharmacy,East Hospital affiliated to Tongji University,Shanghai 200120,China) |
| Abstract: | Objective: To establish a RP-HPLC method for simultaneous determination of contents of baicalin and ferulic acid in Qinmei granules.Methods: The separation was performed on a Platisial ODS C18 column(250 mm×4.6 mm,5 μm) with the mobile phase consisting of acetonitrile-0.085% phosphoric acid(24∶76)at a flow rate of 1.0 ml/min.The detection wavelength was 321 nm.Results: Baicalin and ferulic acid presented a good linearity in the range of 1.09-109.12 μg/ml(r=0.999 8) and 1.09~108.64 μg/ml(r=0.999 9),respectively.They had good intra-and inter-day precision,stability and reproducibility.Average recovery rates of low,middle and high concentrations of baicalin and ferulic acid in Qinmei granules were(99.69±1.24)%,(96.90±0.28)%,(95.06±0.44)%(n=3) and(95.90±1.13)%,(96.70±0.30)%,(102.52±1.18)%(n=3),respectively.Average contents of baicalin and ferulic acid in 3 batches of Qinmei granule samples were(5.85±0.016) mg/g(n=3) and(90±1.8) μg/g(n=3),respectively.Conclusion: It is an accurate,sensitive,reproducibility selective method for determination of the baicalin and ferulic acid in Qinmei granules. |
| Keywords: | Qinmei granules baicalin ferulic acid content determination chromatography high performance liquid |
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