| 体外培养的成骨细胞与珊瑚复合的实验研究 |
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| 引用本文： | 高全文,董绍忠,陈希哲,张勇杰,杨维东,杨连甲. 体外培养的成骨细胞与珊瑚复合的实验研究[J]. 中国组织工程研究与临床康复, 2003, 7(2): 224-225 |
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| 作者姓名： | 高全文 董绍忠 陈希哲 张勇杰 杨维东 杨连甲 |
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| 作者单位： | 解放军第四军医大学口腔医学院病理科,陕西省西安市,710032 |
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| 基金项目： | 国家自然科学基金资助项目(30070818) |
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| 摘 要： | 目的:观察SD大鼠成骨细胞在珊瑚表面的贴附、伸展及生长情况,并探讨珊瑚作为骨组织工程支架材料与细胞复合植入体内的最佳时间。方法:分离的骨髓基质干细胞用含10％胎牛血清的DMEM培养,5d后加矿化液诱导培养,分化为骨髓基质成骨细胞。将成骨细胞与处理过的珊瑚复合,通过细胞计数检测附着后的细胞生长增殖特性,并在扫描电镜下观察细胞在珊瑚表面的贴附情况。结果:细胞接种后,在材料表面贴附良好,继续增殖。结论:珊瑚的生物相容性良好,细胞贴附后继续保持成骨细胞的表型,作为骨组织工程的支架材料有较好应用前景。
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| 关 键 词： | 骨髓细胞 成骨细胞 生物医学工程 |
| 文章编号： | 1671-5926(2003)02-0224-02 |
| 修稿时间： | 2002-09-13 |
Experimental study on coral combined with SD rat osteoblast in vivo |
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| Gao Quanwen,Dong Shaozhong,Chen Xizhe,Zhang Yong|ie,Yang Weidong,Yang LianjiaGao Quanwen.Dong Shaozhong,Chen Xizhe,Zhang Yongjie,Yang Weidong,Yang Lianpa,Stomatological Hospital,Fourth Military Medical University,Xi' an ,Shared Province,China. Experimental study on coral combined with SD rat osteoblast in vivo[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2003, 7(2): 224-225 |
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| Authors: | Gao Quanwen Dong Shaozhong Chen Xizhe Zhang Yong|ie Yang Weidong Yang LianjiaGao Quanwen.Dong Shaozhong Chen Xizhe Zhang Yongjie Yang Weidong Yang Lianpa Stomatological Hospital Fourth Military Medical University Xi' an Shared Province China |
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| Affiliation: | Gao Quanwen,Dong Shaozhong,Chen Xizhe,Zhang Yong|ie,Yang Weidong,Yang LianjiaGao Quanwen.Dong Shaozhong,Chen Xizhe,Zhang Yongjie,Yang Weidong,Yang Lianpa,Stomatological Hospital,Fourth Military Medical University,Xi' an 710032,Shared Province,China |
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| Abstract: | AIM: To observe the adhesion and growth of SD rat osteoblast on the surface of coral and study the best time on coral combined with SD rat osteoblast in vivo. METHODS: Bone marrow stroma cells were cultured with DMEM containing 10% FBS. After inoculating the cells onto the surface of coral, we surveyed the characteristic of proliferation by cell counting. Using scanning electronic microscope, we observed cellular morphology. RESULTS: Cells could be attached to and extended on the surface of coral, and normally grown, proliferated. Coral could promote cell proliferation. CONCLUSION: The results show that coral has good biocompatibility. With rat bone marrow stroma cells, they can be used as biomaterials in bone tissue engineering. |
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| Keywords: | bone marrow cells osteoblast biomedical engineering |
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