The stability of trifluorothymidine: hydrolysis in buffered aqueous solutions |
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Authors: | M. F. JONES |
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Abstract: | The decomposition of trifluorothymidine in aqueous solution, and borate and phosphate buffers has been studied using an h.p.l.c. method. In aqueous solution accelerated studies demonstrate that a storage life, to 10% loss, of more than 30 years at 4°C is predicted, but steam sterilization causes extensive breakdown. Considerable differences occur in the kinetics and routes of decomposition in borate and phosphate buffers in the pH range 4–8. Hydroxide ion attack producing 5-carboxy-2′-deoxyuridine is suppressed in borate buffers. Complex kinetics of decomposition in phosphate buffer are found and possible explanations suggested. Steam sterilization in buffered solution also leads to unacceptable breakdown. |
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