活血补肾合剂对C57BL/6小鼠皮肤血管新生及毛囊中血管内皮细胞生长因子表达的影响

目的:通过观察C57BL/6小鼠皮肤局部血管新生及毛囊中血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)的表达,探讨活血补肾合剂促进小鼠毛发生长的可能机制。方法:以热松香和石蜡混合物脱毛法诱导C57BL/6小鼠休止期毛囊进入生长期,建立动物模型。90只小鼠随机分为活血补肾合剂组、养血生发胶囊组和模型组。造模后第1天起开始灌药,每天观察各组小鼠皮肤毛发生长情况,并分别于造模后第4、11、17天每组分批处死10只小鼠,病理切片计数拔毛部位的血管数,并以免疫组织化学方法检测毛囊中VEGF表达情况。结果:活血补肾合剂组局部新生血管数第4天时多于模型组(P<0.05),第11天时明显多于养血生发胶囊组和模型组(P<0.05);活血补肾合剂组毛囊中VEGF的表达在第11天和第17天时均明显高于养血生发胶囊组和模型组(P<0.05)。结论:活血补肾合剂可能是通过调节细胞因子水平,起到促进血管新生和毛发生长的作用。

Effects of Huoxue Bushen Mixture on skin blood vessel neogenesis and vascular endothelial growth factor expression in hair follicle of C57BL/6 mice

Objective: To investigate the possible stimulating mechanism of Huoxue Bushen Mixture (HXBSM), a traditional Chinese compound medicine, on hair growth of mice via measuring the variance of skin blood vessel neogenesis and vascular endothelial growth factor (VEGF) expression in the hair follicle. Methods: Hot rosin and paraffin mixture depilation were used to induce C57BL/6 mice hair follicle to enter from telogen into anagen. Ninety C57BL/6 mice were divided into 3 groups randomly: HXBSM group, Yang-xue Shengfa Capsule (YXSFC, another traditional Chinese compound medicine) group and untreated group. The mice were fed with corresponding drugs after modeling. The hair growth of the mice was observed every day. Every ten mice out of each group were executed respectively at day 4, 11 and day 17. Skin blood vessel neogenesis was counted through pathological section and VEGF expression in the hair follicle was measured via immunohistochemical method.Results: The number of local blood vessel neogenisis in the HXBSM group observed was larger than that in the untreated group at day 4 (P<0.05) ; and evidently larger than that in the YXSFC group and the untreated group at day 11 (P<0.05). The expression of VEGF in the hair follicle was distinctively higher than that in the YXSFC group and the untreated group at day 11 and day 17 (P<0.05).Conclusion: HXBSM up-regulates VEGF expression to accelerate blood vessel neogenesis and hair growth.