HPLC法同时测定炙甘草汤中甘草苷、甘草素、异甘草素的含量

目的:建立同时测定炙甘草汤中甘草苷、甘草素、异甘草素含量的方法。方法:采用高效液相色谱法。色谱柱为Waters Sunfire C18(250mm×4.6mm,5μm),流动相为乙腈-0.5%冰醋酸(50:50,V/V),检测波长为278nm,流速为1.0mL·min-1。结果:甘草苷、甘草素、异甘草素的检测浓度分别在1.2～24.0、64.0～1280.0、50.0～1000.0μg·mL-1范围内与各自峰面积积分值呈良好的线性关系(r分别为0.9998、0.9999、0.9999);三者平均加样回收率分别为100.38%、99.45%、97.46%,RSD分别为0.45%、0.53%、0.96%(n=6)。结论:该方法准确、可靠,可为炙甘草汤的质量控制提供检测依据。

Simultaneous Determination of Glycyrrizin, Liquiritin and Isoliquiritigenin in Zhigancao Decoction by HPLC

OBJECTIVE： To establish the method for the content determination of glycyrrizin, liquiritin and isoliquifitigenin in Zhigancao decoction. METHODS： HPLC method was adopted. Waters Sunfire C18（250mm×4.6mm,5μm） column was used with aeetonitrile-0.5% acetic acid （50：50, V/V） as mobile phase at the flow rate of 1.0 mL.min^-1. The detection wavelength was 278 nrn. RESULTS： The linear ranges were 1.2-24.0 μg.mL^-1（r=0.999 8） for glycyrrizin,64.0-1 280.0 μg.mL^-1（r=0.999 9） for liquiritin and 50.0-1000.0 μg. mL^-1 （r= 0.999 9） for isoliquiritigenin. The average recovery rates were 100.38 % （RSD=0.45 %, n=6）, 99.45% （RSD=0.53% ,n=6） and 97.46% （RSD=0.96% ,n=6）. CONCLUSION： The method is accurate and reliable for the quality control of Zhigancao decoction.