HPLC-ELSD法测定山茱萸萜中齐墩果酸与熊果酸的含量

目的:建立测定山茱萸萜中齐墩果酸与熊果酸含量的方法。方法:采用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法。色谱柱为Welchrom-C18(250mm×4.6mm,5μm),流动相为甲醇-1%醋酸铵(85∶15,V/V),流速为0.8mL·min-1,柱温为30℃,ELSD检测器的漂移管温度为90℃,载气流量为2.4L·min-1。结果:齐墩果酸、熊果酸的检测浓度分别在15.00～75.00、60.00～300.00μg·mL-1范围内与各自峰面积积分值呈良好的对数线性关系(r均为0.9999);二者平均加样回收率分别为99.14%、97.37%,RSD分别为1.46%、1.79%(n均为6)。结论:本方法准确、可靠,可用于山茱萸萜的质量控制。

Content Determination of Oleanolic Acid and Ursolic Acid in Terpene of Cornus officinalis by HPLC-ELSD

OBJECTIVE：To establish a method for the content determination of oleanolic acid and ursolic acid in terpene of Cornus officinalis.METHODS：HPLC-ELSD method was adopted.The determination was performed on welchrom-C 18 column（250 mm×4.6 mm,5 μm）with mobile phase consisted of methanol-1% ammonium acetate（85：15,V/V）at the flow rate of 0.8 mL.min-1 and column temperature of 30 ℃.The temperature of drift tube was 90 ℃,and flow rate was 2.4 L.min-1.RESULTS：The linear range was 15.00～75.00 μg.mL-1 for oleanolic acid and 60.00～300.00 μg.mL-1 for ursolic acid（r=0.999 9）;their average recovery rates were 99.14%（RSD=1.46%,n=6）and 97.37%（RSD=1.79%,n=6）.CONCLUSION：The method is accurate and reliable.It can be used for quality control of terpene of C.officinalis.