| 乙酰氧基胡椒酚乙酸酯对人乳腺癌MCF-7细胞凋亡与侵袭的影响 |
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| 引用本文： | 刘琳,申莉,华海婴,叶启霞,张明智.乙酰氧基胡椒酚乙酸酯对人乳腺癌MCF-7细胞凋亡与侵袭的影响[J].中国药房,2012(35):3282-3284. |
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| 作者姓名： | 刘琳 申莉 华海婴 叶启霞 张明智 |
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| 作者单位： | [1]郑州大学附属郑州中心医院,郑州450007 [2]郑州大学护理学院,郑州450052 [3]郑州大学医药科学研究院,郑州450052 [4]郑州大学第一附属医院肿瘤科,郑州450052 |
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| 基金项目： | 郑州市科技攻关计划项目 |
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| 摘 要： | 目的:研究乙酰氧基胡椒酚乙酸酯(ACA)对人乳腺癌MCF-7细胞凋亡与侵袭的影响。方法:通过RT-PCR法检测ACA对人乳腺癌MCF-7细胞B细胞淋巴瘤/白血病-2(Bcl-2)、Bcl-2相关X蛋白(Bax)基因表达的影响,免疫细胞化学法检测细胞中基质金属蛋白酶(MMP)-2和MMP-9的表达。结果:MCF-7细胞经50、100、150μmol·L-1ACA干预后,Bcl-2 mRNA表达显著下降,Bax mRNA表达显著升高(P<0.01);50、100、150μmol·L-1ACA作用后细胞中MMP-2和MMP-9的蛋白表达显著减少(P<0.01)。结论:ACA诱导的Bcl-2 mRNA表达的下调和Bax mRNA表达的上调可能参与了其诱导人乳腺癌MCF-7细胞凋亡作用。ACA能抑制人乳腺癌MCF-7细胞中MMP-2和MMP-9的蛋白表达从而抑制其侵袭。
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| 关 键 词： | 乙酰氧基胡椒酚乙酸酯 人乳腺癌MCF-7细胞 凋亡 侵袭 |
Effects of ACA on Apoptosis and Invasion of Human Breast Carcinoma Cell Line MCF-7 |
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| LIU LinZhengzhou Central Hospital of Zhengzhou University,Zhengzhou,China SHEN LiNursing College of Zhengzhou University,Zhengzhou,China HUA Hai-ying,YE Qi-xiaAcademy of Medical and Pharmaceutical Sciences,Zhengzhou University,Zhengzhou,China ZHANG Ming-zhi.Effects of ACA on Apoptosis and Invasion of Human Breast Carcinoma Cell Line MCF-7[J].China Pharmacy,2012(35):3282-3284. |
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| Authors: | LIU LinZhengzhou Central Hospital of Zhengzhou University Zhengzhou China SHEN LiNursing College of Zhengzhou University Zhengzhou China HUA Hai-ying YE Qi-xiaAcademy of Medical and Pharmaceutical Sciences Zhengzhou University Zhengzhou China ZHANG Ming-zhi |
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| Institution: | LIU Lin(Zhengzhou Central Hospital of Zhengzhou University,Zhengzhou 450007, China) SHEN Li(Nursing College of Zhengzhou University, Zhengzhou 450052, China) HUA Hai-ying, YE Qi-xia(Academy of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450052, China) ZHANG Ming-zhi(Dept. of Oncology, The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052, China) |
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| Abstract: | OBJECTIVE： To study the effects of ACA on the apoptosis and invasion of human breast carcinoma cell line MCF-7. METHODS： The effects of ACA on the expression of Bcl-2 and Bax mRNA in MCF-7 cells were detected by RT-PCR. The expression of MMP-2 and MMP-9 were determined by immunocytochemical method. RESULTS： With the treatment of ACA （ 50,100 ,150 μmol. L^-1l）, the expression of Bcl-2 mRNA was down-regulated while the expression of Bax mRNA was up-regulated significantly. （50,100,150 μmol. L^-1） ACA decreased the expression of MMP-2 and MMP-9 in MCF-7 cells. CONCLUSION： ACA exhibits apoptosis induction, which might be related to the down-regulation of Bcl-2 mRNA and up-regulation of Bax mRNA, and ACA inhibits cellular invasion through decreasing the expression of MMP-2 and MMP-9 in MCF-7. |
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| Keywords: | ACA human breast carcinoma cell line MCF-7 Apoptosis Invasion |
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