| 高效液相色谱法测定豆制品中酸性橙Ⅱ和酸性金黄 |
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| 引用本文： | 卢彩霞,陈艳,李永波. 高效液相色谱法测定豆制品中酸性橙Ⅱ和酸性金黄[J]. 实用医技杂志, 2006, 13(15): 2594-2595 |
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| 作者姓名： | 卢彩霞 陈艳 李永波 |
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| 作者单位： | 1. 洛南县疾病预防控制中心,陕西,洛南,726100
2. 西安市疾病预防控制中心,陕西,西安,710002 |
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| 摘 要： | 本文探讨高效液相色谱法测定豆制品中酸性橙Ⅱ和酸性金黄含量的方法,豆制品中酸性金黄Ⅱ经粉碎,提取,过滤,得到的样品提取液用高效液相色谱测定,流动相是甲醇乙酸铵0.02mol/L=75+25,在检测波长450nm时,流速1.0ml/min测定,色谱柱为SymmetryC18(250mm×4.6mmid,5μm)。
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| 关 键 词： | 豆制品 酸性金黄Ⅱ 高效液相色谱 |
| 文章编号： | 1671-5098（2006）15-2594-02 |
| 收稿时间： | 2006-04-13 |
High Performance Liquid Chromatography Determinate of Acid Orange Ⅱ and Orarge Bin Bean Sample |
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| LU Cai-xia,CHEN Yan,LI Yong-bo. High Performance Liquid Chromatography Determinate of Acid Orange Ⅱ and Orarge Bin Bean Sample[J]. Journal of Practical Medical Techniques, 2006, 13(15): 2594-2595 |
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| Authors: | LU Cai-xia CHEN Yan LI Yong-bo |
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| Abstract: | A sensitive and accurate High Performance Liquid Chromatography determinate of Acid OrangeⅡand orange B in bean sample.Acid OrangeⅡand orange B were extract from bean sample with the HPLC method was used to analyze estradiel.The optimized detection parameters were as follows:the colum was Symmetry C18 (250 mm×4.6 mmid,5 μm),the mobile phase was methanol-aninoacetate (0.02 mmol/ml)=75:25,The phase flow rate was 1.0 ml/min.The UV detector was set at 450 nm. |
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| Keywords: | Bean product Acidity gold High performance liquid Chromatography |
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