| 蚯蚓纤溶酶的分离纯化研究 |
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| 引用本文： | 李菁华,赵伟. 蚯蚓纤溶酶的分离纯化研究[J]. 中国生化药物杂志, 2005, 26(5): 288-291 |
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| 作者姓名： | 李菁华 赵伟 |
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| 作者单位： | 北京联合大学,生物化学工程学院,北京,100023 |
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| 摘 要： | 目的从赤子爱胜蚓(Eisenia Foelide)中分离纯化出一种相对分子质量(Mr)较小的纤维蛋白溶解组分———蚯蚓纤溶酶(EFE),为其注射剂的研制开发打下基础。方法采用盐析、透析、凝胶过滤色谱、离子交换色谱等方法分离纯化EFE,用SDS-PAGE对纯化的活性组分进行Mr测定,用酶谱分析方法初步探讨蚯蚓中存在的其它纤溶酶活性组分。结果分离纯化得到了EFE单一组分,经SDS-PAGE EFE呈单一条带,Mr约为25 000。经酶谱分析发现纤溶酶活性组分Mr分布在25 000~50 000之间。结论反复使用色谱技术可分离纯化EFE,并得到单一组分。
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| 关 键 词： | 蚯蚓纤溶酶 凝胶过滤色谱 离子交换色谱 酶谱分析 |
| 文章编号： | 1005-1678（2005）05-0288-04 |
| 收稿时间： | 2004-12-22 |
| 修稿时间： | 2005-03-21 |
Study on the separation and purification of earthworm fibrinolytic enzyme |
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| LI Jing-hua,ZHAO Wei. Study on the separation and purification of earthworm fibrinolytic enzyme[J]. Chinese Journal of Biochemical Pharmaceutics, 2005, 26(5): 288-291 |
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| Authors: | LI Jing-hua ZHAO Wei |
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| Affiliation: | Bio-chemieal Engineering School Beijing Union University, Beijing 100023, China |
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| Abstract: | PurposeTo separate and purify a relatively low molecular weight(M_r) fibrinolytic enzyme(EFE) from Eisenia Foelide.MethodsEFE was purified through extraction salting-out,dialysis,gel filtration,ion exchange chromatography.The M_r of EFE was determined by SDS-PAGE and other active fibrinolytic components were analized by zymogram protocol.ResultsThe M_r of the active components of EFE was mainly between 25 000～50 000.A single fraction EFE,with M_r of 25 000 was obtained.ConclusionA single fraction EFE was obtained by repeated chromatography. |
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| Keywords: | earthworm fibrinolytic enzyme gel filtration chromatography ion exchange chromatography zymogram protocol |
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