| L783V/3T3细胞系的部分生物学特性观察 |
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| 引用本文： | 吕彪,季文琴,程立,黄春妹,宋后燕.L783V/3T3细胞系的部分生物学特性观察[J].中国比较医学杂志,1991(Z1). |
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| 作者姓名： | 吕彪 季文琴 程立 黄春妹 宋后燕 |
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| 作者单位： | 上海医科大学
(吕彪，季文琴，程立，黄春妹)，上海医科大学(宋后燕) |
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| 基金项目： | 国家自然科学基金(88-3880400号)资助 |
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| 摘 要： | 实验结果表明:感染了小鼠白血病病毒的L783V/3T3细胞的形态和生长方式未发生改变,细胞周期各时相细胞比例无明显变化,软琼脂培养未形成集落,制备国L783V/3T3的无细胞提取液,接种于昆明种新生乳鼠,能诱发淋巴细胞白血病。这说明,L783小鼠白血病病毒基因组中不含有致癌病毒基因,此病毒属于慢性致瘤性逆转录病毒。
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| 关 键 词： | 小鼠白血病病毒 软琼脂培养 流式细胞分析 细胞系 |
Biological Characteristic Studies on L783V/3T3 Cell Line |
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| Lu Biao,et al.Biological Characteristic Studies on L783V/3T3 Cell Line[J].Chinese Journal of Comparative Medicine,1991(Z1). |
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| Authors: | Lu Biao |
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| Abstract: | The biological characteristics of L783V/3T3 cell line were investigated. It was demonstrated that L783V/3T3 cells had no morphological and growth changes and no colonies formed in soft agar medium. Lymphocytic leukemia could be induced by inoculation of cell-free extracts from L783V/3T3 cells into Kunming strain newborn mice. The results indicated that there was no oncogene in L783-MuLV genome, L783-MuLV belonged to slow oncogenic virus. |
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| Keywords: | Murine leukemia virus Soft agar medium Flow cytometry Cell line |
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