Nanodesign of new self-assembling core-shell gellan-transfersomes loading baicalin and in vivo evaluation of repair response in skin |
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| Authors: | Maria Manconi Maria Letizia Manca Carla Caddeo Donatella Valenti Claudia Cencetti Octavio Diez-Sales Amparo Nacher Silvia Mir-Palomo Maria Carmen Terencio Davide Demurtas Juan Carmelo Gomez-Fernandez Francisco José Aranda Anna Maria Fadda Pietro Matricardi |
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| Affiliation: | 1. Dept. of Scienze della Vita e dell''Ambiente, University of Cagliari, Cagliari, Italy;2. Dept. of Drug chemistry and technologies, Sapienza, University of Roma, Roma, Italy;3. Dept. of Pharmacy and Pharmaceutical Technology and Parasitology, University of Valencia, Burjassot, Valencia, Spain;4. Institute of Molecular Recognition and Technological Development, Inter-University Institute from Polytechnic University of Valencia and University of Valencia, Spain;5. Dept. of Pharmacology, University of Valencia, Burjassot, Valencia, Spain;6. Interdisciplinary Center for Electron Microscopy, Ecole Polytechnique Fédérale de Lausanne, Station 12, Lausanne, Switzerland;7. Dept. of Biochemistry and Molecular Biology A, Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, Murcia, Spain |
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| Abstract: | Gellan nanohydrogel and phospholipid vesicles were combined to incorporate baicalin in new self-assembling core-shell gellan-transfersomes obtained by an easy, scalable method. The vesicles were small in size (~107 nm) and monodispersed (P.I. ≤ 0.24), forming a viscous system (~24 mPa/s) as compared to transfersomes (~1.6 mPa/s), as confirmed by rheological studies. Gellan was anchored to the bilayer domains through cholesterol, and the polymer chains were distributed onto the outer surface of the bilayer, thus forming a core-shell structure, as suggested by SAXS analyses. The optimal carrier ability of core-shell gellan-transfersomes was established by the high deposition of baicalin in the skin (~11% in the whole skin), especially in the deeper tissue (~8% in the dermis). Moreover, their ability to improve baicalin efficacy in anti-inflammatory and skin repair tests was confirmed in vivo in mice, providing the complete skin restoration and inhibiting all the studied inflammatory markers. |
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| Keywords: | Transfersomes Gellan SAXS analysis Skin delivery Rheological studies Corresponding author at: Dept. of Scienze della Vita e dell'Ambiente, University of Cagliari, 09124 Cagliari, Italy. |
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