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Multimodal doxorubicin loaded magnetic nanoparticles for VEGF targeted theranostics of breast cancer |
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| Authors: | Alevtina S. Semkina Maxim A. Abakumov Alexander S. Skorikov Tatiana O. Abakumova Pavel A. Melnikov Nadejda F. Grinenko Sergey A. Cherepanov Daniil A. Vishnevskiy Victor A. Naumenko Klavdiya P. Ionova Alexander G. Majouga Vladimir P. Chekhonin |
| Affiliation: | 1. Pirogov Russian National Research Medical University, Moscow, Russian Federation;2. The National University of Science and Technology MISiS, Moscow, Russian Federation;3. Lomonosov Moscow State University, Moscow, Russian Federation;4. Skolkovo Institute of Science and Technology, Moscow, Russian Federation;5. Serbsky National Medical Research Center for Psychiatry and Narcology, Moscow, Russian Federation;6. Dmitry Mendeleev University of Chemical Technology of Russia, Moscow, Russian Federation |
| Abstract: | In presented paper we have developed new system for cancer theranostics based on vascular endothelial growth factor (VEGF) targeted magnetic nanoparticles. Conjugation of anti-VEGF antibodies with bovine serum albumin coated PEGylated magnetic nanoparticles allows for improved binding with murine breast adenocarcinoma 4T1 cell line and facilitates doxorubicin delivery to tumor cells. It was shown that intravenous injection of doxorubicin loaded VEGF targeted nanoparticles increases median survival rate of mice bearing 4T1 tumors up to 50%. On the other hand magnetic resonance imaging (MRI) of 4T1 tumors 24 h after intravenous injection showed accumulation of nanoparticles in tumors, thus allowing simultaneous cancer therapy and diagnostics. |
| Keywords: | magnetic nanoparticles drug delivery chemotherapy MRI-contrast agents breast adenocarcinoma iron oxide BSA bovine serum albumin MNP PEGylated albumin coated magnetic nanoparticles VEGF vascular endothelial growth factor Dox doxorubicin MRI magnetic resonance imaging AES atomic emission spectrometry CNR Contrast to Noise Ratio mAb monoclonal antibody TEM transmission electron microscopy AFM atomic force microscopy EDC 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide NHS N-hydroxysuccinimide ELISA enzyme linked immunosorbent assay |
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