新型四物胶囊对辐射损伤小鼠造血功能的影响

目的评价新型四物胶囊对60Coγ射线所致小鼠造血功能损伤的影响,为该药的临床研究提供参考依据。方法采用2.5 Gy60Coγ射线全身照射,照后连续给药7 d。正常组和模型组给予等体积的蒸馏水,新型四物胶囊高、中、低剂量（20.0、10.0、5.0 g.kg-1）组分别在照前、照后第1、4、7、11、14、17、23天检测外周血象,同时在照后第7天进行CD34＋细胞检测、骨髓造血祖细胞集落培养。结果新型四物胶囊各剂量在造模后7 d提高CD34＋细胞数,促进CFU-GM增殖,同时高剂量还促进CFU-E、BFU-E及CFU-Mix增殖;第7～14天显著升高白细胞与血小板数。结论新型四物胶囊可有效改善60Coγ射线对小鼠所致的造血损伤,提高骨髓造血功能,进而促进外周血各组分恢复正常。

Effect of New Si-wu Capsules on Hematopoietic Function of Irradiated Mice

Objective To evaluate the hematopoietic function of new Si-wu capsules on low-dose 60Coγirradiated mice. Methods Balb/c mice were irradiated using 60Coγresources. Following irradiation, the extract of new Si-wu capsules was administered intragastrically for 7 consecutive days at doses of 20. 0,10. 0,5.0 g .kg-1. The normal group and model group were given an equal volume of distilled water. Blood samples were collected from the tail end and the number of peripheral blood cells was counted on day 0,1,4,7,11,14,17 and 23 using a microcell counter. The ability of the colony-forming unit of bone marrow cells and the percentage of CD34 ＋ cells in bone marrow were observed. Results Extracts of new Si-wu capsules could increase the percentage of CD34 ＋ cells and the ability of the colony-forming unit of granulocyte macrophage. High doses of new Si-wu capsules treatment could also improve the ability of erythroid and mixed colony-forming units, new Si-wu capsule could accelerate the recovery of white blood cells and platelets. Conclu- sion new Si-wu capsule can reverse hematopoietic injury induced by 2.5 Gy 60Coγ irradiation ,improve the hematopoietic function of bone marrow and accelerate blood cell recovery.