通腑活血汤对脑出血大鼠脑组织保护作用的实验研究

目的:探讨通腑活血汤抗脑出血的作用机制。方法:采用Ⅶ型胶原酶诱导大鼠脑出血,建立脑出血模型。分假手术组,模型组,脑血康组,通腑活血汤组,各组每天灌胃2次,每次3 mL。观察神经缺损症状的变化及检测脑系数、脑含水量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、一氧化氮(NO)含量。结果:模型组大鼠偏瘫状态明显,脑系数、脑含水量增高,脑组织出现水肿,说明胶原酶诱导的大鼠脑出血模型成功。通腑活血汤组能明显改善实验大鼠的偏瘫程度(P<0.05)及减少脑系数、脑含水量和MDA含量;提高SOD活性和NO含量(P<0.05)。结论:通腑活血汤对于脑出血动物模型上述指标具有明显的改善作用,具有抗脑出血大鼠氧自由基作用。

Experiment study of Tongfu Huoxue decoction in the treatment of intracelebral hemorrhage

OBJECTIVE: To investigate the effects of Tongfu Huoxue decoction on experimental intracelebral hemorrhage and the associated machenisms. METHOD: The cerebral hemorrhage model in rats was induced by local injection of type VII collagenase and they were randomly divided into four groups. The treated groups were treated with Naoxuekang and Tongfu Huoxue decoction. The control groups were only treated with water. The changes of neurological defect were observed. The content of brain water, MDA, NO and the activity of SOD were measured. RESULT: The cerebral hemorrhage rats showed hemiplegia, and the hemorrhage brains showed celebral edema, higher quotient of brain and content of brain water, suggesting the hemorrhage model was established successfully. After the treatment of Tongfu Huoxue decoction, the hemorrhage rats showed smaller hemorrhage volume, the brain tissue from the hemorrhage rats had lower MDA content and the quotient of brain, and also had higher activity of SOD and content of NO. CONCLUSION: Tongfu Huoxue decoction has treatment effects on cerebral hemorrhage.