| HPLC波长切换法测定莪术残油中莪术二酮莪术醇和吉马酮的含量 |
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| 引用本文： | 孙艳涛,刘妹畅,张振秋.HPLC波长切换法测定莪术残油中莪术二酮莪术醇和吉马酮的含量[J].中医药学刊,2010(2):391-392. |
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| 作者姓名： | 孙艳涛 刘妹畅 张振秋 |
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| 作者单位： | 辽宁中医药大学药学院,辽宁大连116600 |
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| 摘 要： | 目的：建立用HPLC波长切换法同时测定莪术残油中莪术二酮、莪术醇和吉马酮含量的测定方法。方法：采用Kromasil 5μm C18（4.6mm×150mm）;流动相为乙腈-1%磷酸溶液;流速1.0 mL.min-1;检测波长213 nm,236nm;柱温35℃。结果：莪术二酮在0.1215～0.6076μg范围内线性关系良好,平均回收率为100.0%,RSD为2.4%;莪术醇在0.5760～2.880μg范围内线性关系良好,平均回收率为99.9%,RSD为2.1%;吉马酮在2.014～10.07μg范围内线性关系良好,平均回收率为100.3%,RSD为2.3%。结论：不同批号的莪术残油中3种成分的含量差异不显著。
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| 关 键 词： | 莪术油 莪术二酮 莪术醇 吉马酮 高效液相色谱法 |
Determination of Curdione Curcumol and Germacrone of Residual Zedoary oil by HPLC |
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| SUN Yan-tao,LIU Shu-chang,ZHANG Zhen-qiu.Determination of Curdione Curcumol and Germacrone of Residual Zedoary oil by HPLC[J].Study Journal of Traditional Chinese Medicine,2010(2):391-392. |
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| Authors: | SUN Yan-tao LIU Shu-chang ZHANG Zhen-qiu |
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| Institution: | (College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,Liaoning,China) |
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| Abstract: | Objective：To establish the method of determine the content of Curdione,Curcumol and Germacrone in residual Zedoary oil by HPLC.Methods： Kromasil 5μm C18（4.6mm×150mm）;mobile phase of acetonitrile-1% phosphoric acid;flow rate of 1.0 mL ·min-1;detection wavelength 213 nm,236nm;Column temperature： 35℃.Results： Curdione 0.1215～0.6076μg at the scope of a good linear relationship,the average recovery 100.0%,RSD 2.4%;Curcumol 0.5760～2.880μg at the scope of a good linear relationship,the average recovery 99.9%,RSD 2.1%;Germacrone at the scope of 2.014～10.07μg a good linear relationship,the average recovery 100.3%,RSD 2.3%.Conclusion： Different batch of residual zedoary oil composition of three kinds of content not significantly. |
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| Keywords: | zedoary turmeric oil curdione curcumol germacrone HPLC |
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