人工种子在盾叶薯蓣快速繁殖技术中的初步应用

为提高盾叶薯蓣试管苗的移栽成活率,在组织培养的基础上,对盾叶薯蓣人工种子中人工种皮、人工胚乳制作,人工种子一次性成苗及移栽成活率等问题进行了初步研究,结果表明,人工种子快速繁殖的最适条件为：从盾叶薯蓣愈伤组织表面切取2～3mm小块茎,包埋在2.5%海藻酸钠里,滴入2%CaCl2中反应8～10min,人工胚乳MS/2＋IAA0.4mg/L直接播种在MS/2培养基上,一次性成苗率可达100%左右,移栽成活率高达85%。说明以组织培养为基础的盾叶薯蓣人工种子快速繁殖技术具有一定的优越性。

The Elementary Application of Artificial Seeds in the Technique of Rapid Multiplication of of D. zingibiernsis

To improve the surviving rate of the plantlets of D.zingibiernsis,on the basis of tissue culture,The technique of artificial endosperm,artificial seeds of D.zingibiernsis,the germination ratio of artificial seeds and the surviving rate were investigated.The results demonstrated that the condition of rapid multiplication of artificial seeds should be as following：①Cutting about 2～3mm size embryo coming from callus.②Cultured embryo were encapsulated with 2.5% solidumalginate in IAA 0.4mg/L solution and then dropped in 2%CaCl2 solution for 8～10min.3） Artificial seeds were germinated on the MS/2 media.According to the above procedure,the germination ratio of artificial seeds could reach 100% or so and the surviving rate could reach 85% or so.Therefore,the technique of rapid multiplication of artificial seeds on the basis of tissue culture of D.zingibiernsis had definite superiority.