肾病I号方对大鼠系膜细胞增殖及CTGFCOL-Ⅳ表达的影响

目的：观察肾病Ⅰ号方（SBYHF）对体外培养的大鼠肾小球系膜细胞（glomerular mesangial cell, GMC）的增殖及表达IV型胶原（COL-Ⅳ）及结缔组织生长因子（CTGF）的影响,探讨其抗肾纤维化的机制。方法：通过体外培养GMC技术及体外药物血清实验方法 ,采用CCK-8法检测GMC的增殖,ELISA法检测COL-Ⅳ的分泌水平,RT-PCR法检测CTGFmRNA的表达。结果：肾病Ⅰ号方可显著抑制GMC的增殖（P〈0.01）,减少COL- Ⅳ的分泌和CTGF mRNA的表达（P〈0.01）。结论：肾病Ⅰ号方可能通过减少GMC的COL- Ⅳ的分泌,下调CTGF mRNA表达,而抑制GMC增殖,从而发挥抗肾脏纤维化的作用。

The Effects of SBYHF on Proliferation and Expression of COL- IV and CTGF in Rat Glomerular Mesangial Cells

Objective： To explore the molecular and cellular mechanism of SBYHF in ameliorating renal fibrosis through observing the effect of proliferation andexpression ofCOL-IV and CTGFin Rat Glomerular Mesangial Cells.Methods： The proliferation of Rat GMC fostered in SBYHF was detected by CCK-8, the COL-IV secretion was detected by ELISA, and the CTGF was detected by Real Time PCR. Results： SBYHF significantly inhibited GMC proliferation（P〈0.01） and decreased the expression of COL-IV and CTGF （P〈0.01）. Conclusion： SBYHFmay through reduces the COL-IV secretion and down-regulated the expression ofCTGF to inhibit the proliferation of Rat Glomerular Mesangial Cells. This may be the mechanism of SBYHF in ameliorating renal fibrosis.