| 水蛭 地龙药材商品中总多糖含量测定 |
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| 引用本文： | 丁冠华,李峰,康廷国.水蛭 地龙药材商品中总多糖含量测定[J].中医药学刊,2010(5):986-987. |
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| 作者姓名： | 丁冠华 李峰 康廷国 |
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| 作者单位： | 辽宁中医药大学药学院,辽宁大连116600 |
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| 基金项目： | 国家自然科学基金资助项目（30973881）; 沈阳市科技局资助项目（1081238-1-00） |
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| 摘 要： | 目的：建立水蛭、地龙两种药材中总多糖含量测定方法,以评价此两种商品药材质量。方法：采用乙醚回流提取,苯酚-硫酸显色,分光光度法分别测定两种药材不同商品中总多糖的含量。结果：各样品中总多糖含量以葡萄糖计,在18.54～92.68μg范围内有良好线性关系,回归方程Y=0.0045X＋0.0012（r=0.9998）。结论：采用苯酚-硫酸法,测定水蛭、地龙药材商品中总多糖含量,方法简便、可靠,可作为水蛭、地龙药材质量控制的方法。
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| 关 键 词： | 水蛭 地龙 总多糖 含量测定 苯酚-硫酸法 |
Determining Beterming the Content of Total Polysaccharide in Hirudo and Pheretima |
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| PING Guan-hua,LI Feng,KANG Ting-guo.Determining Beterming the Content of Total Polysaccharide in Hirudo and Pheretima[J].Study Journal of Traditional Chinese Medicine,2010(5):986-987. |
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| Authors: | PING Guan-hua LI Feng KANG Ting-guo |
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| Institution: | (Phamacy College of Liaoning University of TCM,Dalian 116600,Liaoning,China) |
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| Abstract: | Objective：To establish the method for determining the content of total polysaccharide in Hirudo Pheretima and evaluate the quality of Hirudo Pheretima.Method：After circumfluence-extracting by phenol-vitriolic acid agent display color and adopting Ui-vis to determine the content of total polysaccharide in Hirudo Pheretima.Result：The content of total polysaccharide in Hirudo Pheretima have calculated by glucose.It has good linear correlation 18.54～92.68μg,and the linear equation of polysaccharide is Y=0.0045X＋0.0012（r=0.9998）.Conclusion：The above method to determine the content of total polysaccharide in Hirudo Pheretima is brief,the result of methodology is ideal,it can pilot quality control of the Hirudo Pheretima. |
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| Keywords: | Hirudo Pheretima total polysaccharide assaying phenol-vitriolic acid method |
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