温郁金对人胃癌裸鼠原位移植瘤中MVD表达的影响

目的：通过建立人胃癌裸鼠原位称植瘤模型,研究温郁金对人胃癌裸鼠原位移植瘤生长转移的抑制作用及对MVD表达的影响。方法：BALB/c裸小鼠12只,以荷SGC-7901人胃癌细胞株裸鼠皮下移植瘤为材料,通过手术将癌组织转移到裸小鼠胃壁建立胃癌原位称植模型;原位移植24h后开始灌胃。当动物出现明显消瘦、弓背、神萎等衰竭征象（约9周）时处死动物,观察和比较两组肿瘤抑制率和肿瘤转移情况。用免疫组织化法检测两组移植瘤瘤体中MVD的表达。结果：所有荷瘤鼠胃壁均有肿瘤生长,治疗组瘤重明显低于对照组,P〈0.01,抑瘤率为42%;模型组腹膜转移6/6、肝脏转移5/6、腹水1/6,治疗组腹膜转移2/6、肝脏转移1/6、腹水0/6,其中腹膜及肝脏转移情况差异具有统计学意义（P〈0.05）。温郁金治疗组瘤体中MVD的表达明显下调。结论：温郁金可能通过减少瘤体内MVD抑制人胃癌裸鼠原位移植瘤生长和转移。

Inhibition of Common Turmeric to the Growth and Metastasis of Nude Mouse Orthotopic Implant/metastatic Model of Human Gastric Cancer and its Effect on the Expression of Microvessel Density

Objective：To investigate the effects ofcommon turmeric（wenyujin） on the nude mouse model of human gastric carcinoma cells using orthotopic transplantation and its effect on the expression of Microvessel density.Methods：Firstly we established nude mouse orthotopic implant/metastatic model of human gastric cancer.Human gastric adenocarcinoma cell line（SGC-7901）was inoculated subcutaneously into nude mice to develop implant tumor.Intact tumor tissue was then collected and implanted orthotopically into the stomach of nude mouse.They were administrated after 24 hour.When they became sick about nine weeks later,they were killed.The inhibitary rates of ttltnour growth were detected,and local invasiveness,the rates of metastasis in liver and peritoneum were compared between the two groups.By immunohistochemical method,the expression of Microvessel density in gastric carcinoma were determined.Resuits：The rate of successfully orthotopic transplantation was 100%.Theweightof the tumors in the experimental group was less thanthat ofthe control group significantly（P0.01）,The inhibitary rates ofthe experimental group was 42%.In the face oftumor metastasis.The rates of metastasis in peritoneum,liver and ascites of the control group were 6/6,5/6,1/6,those in experimental group were 2/6,1/6,0/6,respectively.Compared with the control group,the rates of metastasis of peritoneum,liver in the experimental group were significantly lower（P0.05）.meanwhile we found that the posrive rate of MVD in the experimental groups were obviously lower than those in control group（P0.05）.Conclusions：Common turmeric has a strong mhibitory effect on both growth and metastasis of gastric cancer and the expression of Microvessel density.