肾虚对雄性小鼠及其雄性仔鼠精子DNA损伤的影响

目的：观察肾虚对雄性小鼠及其雄性仔鼠精子DNA损伤的影响。方法：将30只雄性昆明小鼠用计算机取随机数法随机分为正常对照组、模型组和补肾组3组。其中模型组和补肾组采用房劳加惊恐的复合伤肾法制造肾精亏虚模型。从造模结束次日起,所有小鼠与正常发情雌鼠配对饲养5天后,处死,取附睾精子,同时进行精子单细胞凝胶电泳（Single cell gel electropherosis,SCGE）、精子染色体结构分析（sperm chromosome structure analysis,SCSA）检测其精子DNA损伤。取出雌鼠待其分娩。仔鼠饲养至6周龄时每组随机取出10只雄性仔鼠,用SCGE和SCSA检测其精子DNA损伤。结果：模型组小鼠与正常组相比精子细胞拖尾率（40.51±11.21vs23.64±9.86,P〈0.01）和DFI（37.42±7.19vs17.11±4.98,P〈0.01）均明显升高,补肾组（拖尾细胞率25.31±8.59,DFI24.31±7.99,P〉0.05）虽然存在DNA损伤,但与正常组相比没有明显差异。模型组仔鼠精子细胞拖尾率（24.59±9.45vs18.53±8.51P〈0.05）及DFI（32.18±9.90vs17.29±5.14,P〈0.01）亦明显较正常组高,但补肾组仔鼠精子DNA损伤程度较轻（拖尾细胞率20.61±6.34,DFI15.22±7.49,P〉0.05）,与正常组相比没有明显差异。结论：经＂惊恐、房劳＂方法造模后,模型组小鼠精子DNA损伤程度明显上升,而补肾疗法则能较好地保护精子DNA完整性。肾虚雄性仔鼠精子DNA完整性有类似于父代的改变,但程度较轻。说明肾虚造成的生殖系统损害,可能通过DNA损伤影响了其仔鼠的生殖健康。

The Effect of Kidney-jing Deficiency on the Sperm DNA Damage of the Male Mice and Their Offspring

Objective：Researched the affect of insufficient ShenJing on the sperm DNA damage of the male mice and their offspring.Materials and methods.Methods：KunMing mice,6 weeks old,30 males and 330 females,were allocated to 3 groups（blank control group,model group,BuShen group） randomly.The mice of the model group and the BuShen group were processed by the ＂fearness combined with excessive sexual behavior＂ to made the model of.insufficient ShenJing.The mice in BuShen group were lavaged of concentrated solution of BuShenFang,the dosage is 0.16mL/10g based on their weigh.Other groups treat with physiological saline at the same dosage.This phase would continued 21 days.All of 3 groups were matched-pairs with health female mice for 5 days after model making.It was observed the tailed cell rate and DFI（DNA damage index） of male mice and their male offspring.Results：Compared with normal group,tailed cell rate and DFI of model group mice were increased significantly（40.51±11.21 vs 23.64±9.86,P〈0.01,37.42±7.19 vs 17.11 4.98,P〈0.01）.There is no significantly difference between normal group and BuShen group（P〈0.05）.For offspring mice,tailed cell rate and DFI of model group were increased significantly（40.51 11.21 vs 23.64 9.86,P〈0.01,37.42 7.19 vs 17.11 4.98,P〈0.01） compared with normal group,and there is no significantly difference between normal group and BuShen group（P〉0.05）.Conclusion：Fearness combined with excessive sexual behavior could damage the sperm DNA of model mice,and these pathologic change could be transferred to their male offspring.BuShen could interrupt this effect.