阿霉素诱导十二指肠闭锁的实验研究

目的 制作阿霉素诱导大白鼠胎仔出现先天性十二指肠闭锁的模型。方法 Ｗｉｓｔａｒ孕鼠１０只，于妊娠第６至第９天腹腔注射阿霉素１．７５ｍｇ／ｋｇ，妊娠２０天剖宫取胎仔，做解剖、电镜及光镜观察。结果 用药组获胎仔３２只，十二指肠闭锁２６例（８１．３％），其中腔内闭锁１５．４％，闭锁近远端由纤维或胰腺相连者占４６．２％，近远端游离者为３８．５％，十二指肠闭锁畸形均伴有胰腺发育异常，９例胰颈、胰体及胰尾缺如

Congenital Duodenal Atresia in Rat Model Induced by Adriamycin

Objective To describe a reprducible fetal model of duedenal atresia. Methods Themated Pregnant rats were given 1. 75 rug/kg of Adriamycin intraperitoneally on days 6 through 9 of gestation, and the litters were recovered on day 20. The fetuses were dissected microscopically and studied histologically. The findings were compared with those of age-matched saline embryos. Results All saline embryos were normal, whereas 81. 7% (26/32) of Adriamycin ernbryoo had duodenal atresia. The intralumi-nal atresia was found in 15. 4% (4/26) of duedenal atresia embryos, gapped atresia with pancreatic tissuefilled in between in 46. 2% (12/26) and gapped atresia in 38. 5 % (10/26). A double duedenal atresia wasfound in one fetus. The malformation was anatomically identical to that of human human. Interestingly,all duedenal atresia embryco were saaociated with pancreatic agenesis, in 34. 6 % with the pancreatic neck,ho and tail absence and in 65. 4% with the body and tail absence. Conclusion This easily repnducibleexperimental model permits new research into both its embryogenesis and the biology if the duedenal atresiafetus.