酶标金葡菌A蛋白结合物用于猪种布病疫区人群血清流行病学检查的价值

本文介绍了应用HRP-SPA结合物,作为第二抗体做ELISA试验,用于猪种布病疫区人群血清流行病学检查。本方法重复性好,特异性强,敏感性高。用该法对猪种布病疫区1168份人血清进行检查,同时用布病血检常规(SAT、CFT、PAT、RBPT、AGT),进行对比检定,结果表明:无论对人猪种布病的诊断或疫区人群的普查,ELISA的阳性率都比SAT、CFT、RBPT、PAT、AGT高。ELISA与SAT的符合率为92.6％。发现ELISA与SAT之间有良好的正相关;HRP-SPA结合物与HRP-兔抗人IgG结合物敏感性、特异性相似。认为利用HRP-SPA作为第二抗体的ELISA技术,无论对人猪种布病的诊断或疫区人群进行大规模血清流行病学检查都是适用的,有推广的价值。

Evaluation of HRP-SPA combination in ELISA for examining the brucellosis of human in the swine brucellosis epidemic area

This report recommends the use of HRP-SPA combination as a second antibody in ELISA for examining the brucellosis of human in a swine brucellosis epidemic area.This method had a good reiteration, a sharp specificity and a high sensibility. It was applied in the examination of 104 sera of patients who suffered from chronic swine brucellosis and 1064 sera of human in the swine brucellosis area.At the same time, the routine sero-diagnosis methods of brucellosis ( SAT,CFT, PAT, RBPT, AGT)were used to be compared with it. The results demonstrated that ELISA had a positive rate higher than that of SAT, CFT, RBPT, PAT, AGT.