应激对中枢神经系统即刻早期基因c-fos表达及HPA轴的调节作用研究

目的：研究应激对即刻早期基因ｃ－ｆｏｓ表达的调节作用及其与ＣＲＦｍＲＮＡ、ＨＰＡ通路和行为之间的关系。方法：制造社会应激动物模型，高架十字迷宫和空场实验评估大鼠应激行为，免疫组织化学技术检测ＦＯＳ蛋白表达，计算机图像分析系统定量，原位杂交检测ＣＲＦｍＲＮＡ表达，放射免疫分析方法和酶联免疫吸附方法（ＥＬＩＳＡ）检测血清ＡＣＴＨ和Ｃｏｒｔｉｓｏｌ。结果：提示室旁核（ＰＶＮ）部位所ｃ－ｆｏｓ，可能作为第

Effects of stress on Immediate Early Gene c-fos Expression and HPA Axle Regulation in Rats

Objective:to investigate the regulatory mechanism of stress on expression of immediate early gene c-fos and the relation to CRFmRNA,HPA pathway and behavior in rats.Method:anxiety was induced in rats by a social defeat paradigm and assessed immediately by exposure to the cross maze and open field test.The expression of protein product of c-fos was examined by immunochemical technique in different brain regions and quantified by computer assisted image analyzing system.In situ hybridization was used to detect the expression of CRHmRNA.Serum ACTH and cortisol were analyzed by RIA and ELISA.Results:there are significant behavior changes in the social defeated rats showed by cross maze and open field test.The protein product of c-fos was significantly expressed in PVN and amygdala compared to control group(p<0 001).The expression of CRFmRNA was also located in PVN.Serum cortisol was elevated significantly(p<0 05)and serum ACTH was elevated without significance.Conclusion:c-fos in the PVN may be involved in the expression of CRF as third messenger and thereby control the excretion of ACTH,activate the HPA axle and affect the behavior and neuro endocrine immune activity related to stress.