化疗对乳腺癌患者外周血中Treg细胞数目的影响及意义

目的: 研究化疗药物对乳腺癌患者根治术后外周血中Treg(CD₄⁺CD₂₅⁺调节性T细胞)的影响及意义。 方法: 采集52例乳腺癌术后患者化疗前1天及化疗后第10天外周静脉血,应用流式细胞技术检测外周血中Treg细胞以及CD₃⁺、CD₄⁺、CD₈⁺T细胞占T淋巴细胞百分比,采用ELLSA法检测外周血中IL-4、IL-10、TGF-β1和IFN-γ的表达水平。 结果: 化疗后乳腺癌患者外周血中Treg细胞占T淋巴细胞百分比(4.75±1.60)%较化疗前(5.59±1.81)%减少(Plt;0.05),且与淋巴结有无转移、转移数量以及绝经状态无关(P>0.05)。化疗前后CD3⁺细胞占T淋巴细胞比例分别为(64.690±7.469)%,(64.357±9.356)%(<0.05)。化疗前后CD4+T细胞占T淋巴细胞比例分别为(38.048±10.671)%,(36.536±9.664)%(Pgt;0.05)。化疗后CD8⁺T细胞占T淋巴细胞比例(28.129±10.900)%较化疗前(24.876±6.631)%升高(Plt;0.05)。化疗后CD₄⁺/CD₈⁺(1.506±0.691)较化疗前(1.680±0.704)降低(Plt;0.05)。化疗后乳腺癌患者外周血中IL-4、IL-10、TGF-β1浓度较化疗前降低,而IFN-γ浓度增高(P<0.05)。化疗前后Treg细胞比例变化与细胞因子IL-4、IL-10、TGF-β1和IFN-γ浓度变化无关(P>0.05)。 结论: 化疗可使乳腺癌患者外周血中Treg细胞占T淋巴细胞比例降低,从而达到治疗肿瘤的效果。

The Effect of Chemotherapy on the Number of Treg in the Peripheral Blood of Patients with Breast Cancer

Objective: To investigate the effect of chemotherapy on Treg (CD₄⁺CD₂₅⁺ regulatory T cell) in the peripheral blood of patients with breast cancer who received radical mastectomy or modified radical mastectomy. Methods: A total of 52 samples of the peripheral blood of post-operated patients with breast cancer were collected on the 1st day before chemotherapy and the 10th day after chemotherapy. The proportion of Treg and CD₃⁺, CD₄⁺, and CD₈⁺ T cells in the to-tal amount of T cells was evaluated by flow cytometry. The cytokine such as IL-4, IL-10, TGF-β 1 and IFN-γwere mea-sured by ELLSA. Results: The proportion of Treg in the peripheral blood was smaller before chemotherapy than after chemotherapy (P<0.05). The changes in Treg was not correlated with lymph node status, the number of positive lymph nodes and menopause status(P>0.05). No statistical significance was found in the percentage of CD₃⁺ and CD₄⁺ T cells in the peripheral blood between the samples of pre-and post-chemotherapy (P>0.05). The proportion of CD₈⁺ T cells in Tlymphocytes increased from (24.876± 6.631)% before chemotherapy to (28.129± 10.900)% after chemotherapy(P<0.05), but CD₄⁺/CD₈⁺ decreased from (1.680± 0.704)% to (1.506± 0.691)%(P<0.05). The levels of IL-4, IL-10 and TGF-β 1 after chemotherapy were lower than those before chemotherapy (P<0.05), but the level of IFN-γwas higher after chemotherapy (P<0.05). However, the changes in Treg were not correlated with the changes in the level of cytokines such as IL-4, IL-10, TGF-β 1 and IFN-γ. Conclusion: Chemotherapy may inhibit tumor through reducing Treg in the peripheral blood of patients with breast cancer.