| 蒽酮染色法测定维地红涂剂中多糖的含量 |
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| 引用本文： | 余学英,常明泉,陈芳,王刚. 蒽酮染色法测定维地红涂剂中多糖的含量[J]. 药学实践杂志, 2014, 32(5): 352-353,356 |
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| 作者姓名： | 余学英 常明泉 陈芳 王刚 |
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| 作者单位： | 湖北医药学院附属太和医院, 湖北 十堰 442000,湖北医药学院附属太和医院, 湖北 十堰 442000,湖北医药学院附属太和医院, 湖北 十堰 442000,湖北医药学院附属太和医院, 湖北 十堰 442000 |
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| 基金项目： | 太和科研基金资助项目(2009D34). |
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| 摘 要： | 目的建立维地红涂剂中多糖的含量测定方法。方法用无水葡萄糖为对照品,0.2%蒽酮-硫酸试液为染色剂,紫外分光光度法测定,测定波长620 nm。结果多糖在1.9-34.2μg/ml范围内线性关系良好,Y=0.033X-0.006 7（r=0.999 9）,回收率为98.6%,RSD=1.06%（n=6）。结论所建方法便捷、重现性好,适用于维地红涂剂中多糖的含量测定。
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| 关 键 词： | 维地红涂剂 多糖 含量测定 |
| 收稿时间： | 2013-10-12 |
| 修稿时间： | 2013-12-27 |
Determination of the polysaccharide in Verdihong paint |
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| YU Xueying,CHANG Mingquan,CHEN Fang and WANG Gang. Determination of the polysaccharide in Verdihong paint[J]. The Journal of Pharmaceutical Practice, 2014, 32(5): 352-353,356 |
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| Authors: | YU Xueying CHANG Mingquan CHEN Fang WANG Gang |
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| Affiliation: | Taihe Hospital Affiliated to Hubei University of Medicine, Shiyan 442000, China,Taihe Hospital Affiliated to Hubei University of Medicine, Shiyan 442000, China,Taihe Hospital Affiliated to Hubei University of Medicine, Shiyan 442000, China and Taihe Hospital Affiliated to Hubei University of Medicine, Shiyan 442000, China |
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| Abstract: | Objective To establish the determination method of the polysaccharide in Verdihong paint. Methods The anhydrous dextrose was used as the control,0. 2% anthrone-sulfuric acid as the stain,the content of polysaccharide was determined by ultraviolet spectrophotometer at the wavelength of 620 nm. Results The polysaccharide curve was linear within a range of 1. 9 ~ 34. 2μg/ml,linear equation was Y =0. 033 X- 0. 006 7（ r = 0. 999 9）,the average recovery was 98. 6% with RSD of 1. 06%（ n = 6）.Conclusion This method was convenient,stable and coud be used to assay content of the polysaccharide in Verdihong paint. |
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| Keywords: | Verdihong paint polysaccharide content determination |
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