HPLC 法测定双黄滴丸中黄芩苷和绿原酸的含量

目的建立HPLC法测定双黄连滴丸中黄芩苷和绿原酸含量的方法。方法采用C18色谱柱，乙腈-0．1％磷酸溶液为流动相（梯度洗脱），流速为1．0ml-min^-1,检测波长为318nm。结果黄芩苷线性范围为0．1-0．5g·L^-1，平均回收率=98．50％，RSD=1．13％；绿原酸线性范围为0．024-0．12g·L^-1，平均回收率=98．25％，RSD=0．37％。结论该方法简便、可靠、准确，可用于该制剂的质量控制。

Determination of chlorogenic acid and baicalin in shuanghuanglian pill by HPLC

Aim To establish a method of HPLC for determination of baicalin and chlorogenic acid in shuanghuanglian pill.Methods C18 column was used.The acetonitrile-0.1% phosphoric acid solution was used as the mobile phase.The flow rate was 1.0 ml·min-1.The detection wavelength was set as 318nm.Results For baicalin,the linear range was 0.1～0.5 g·L-1,and the average recovery rate was 98.50 % with an RSD of 1.13%.For chlorogenic acid,the linear range was 0.024～0.12 g·L-1,and the average recovery rate was 98.25% with an RSD of 0.37%.Conclusion The method is simple,reliable,accurate and can be applied to the quality control of the preparation.