MiR-124-3p inhibits the migration and invasion of Gastric cancer by targeting ITGB3 |
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| Affiliation: | 1. Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China;2. Department of Clinical Laboratory, Santai People''s Hospital, Santai 621100, Sichuan, China;3. Sichuan Forensic Expertises Center in Southwest China, Chengdu 610041, China;4. Department of Clinical Laboratory, People''s Hospital of Beichuan Qiang Autonomous County, Beichuan 622750, Sichuan, China;5. Department of Forensic Genetics, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China;1. Department of Gynecology, Xiangyang No.1 People''s Hospital, Hubei University of Medicine, 441000 Xiangyang, China;2. Department of Gynaecology, Renmin Hospital of Wuhan University, 430060 Wuhan, China;3. Department of Pathology, Xiangyang Hospital Affiliated to Hubei Medical College, 441000 Xiangyang, China;4. Department of Gynecology, Xiangyang Hospital Affiliated to Hubei Medical College, 441000 Xiangyang, China;1. Clinical Laboratory, Enze Hospital, Taizhou Enze Medical Center, Luqiao 318050, China;2. Clinical Laboratory, Taizhou First People’s Hospital, Huangyan Hospital of Wenzhou Medical University, Huangyan 318020, China |
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| Abstract: | BACKGROUNDGastric cancer is one of the major malignant tumors in the world. Integrins expressed in cancer cells can promote tumor progression and migration. MiRNAs can inhibit the expression of target genes by directly binding to their mRNAs and can affect various important biological processes. The aim of this study was to investigate the role of miR-124- 3p and ITGB3 in gastric cancer.METHODSRT-PCR and western blot are used to detect the expression of miR-124-3p, ITGB3 and integrin β3 in gastric cancer tissues and cells. The wound healing, CCK-8 assay, transwell migration and invasion assay were performed to determine the cell proliferation, migration and invasion. What’s more, bioinformatics prediction and luciferase assay was conducted to demonstrated the binding efficiency between miR-124-3p and ITGB3.RESULTSWe verified that ITGB3 and miR-124-3p changes the migration and invasion of gastric cancer cells in vitro. The overexpression or silencing of miR-124-3p inhibited or promoted the proliferation, migration and invasion of both selected gastric cancer cells, and ITGB3 is just the reverse. Meanwhile, we validated that ITGB3 is the target of miR-124-3p by bioinformatics prediction and luciferase assay. Lastly, the expression of ITGB3 in 40 pairs of gastric cancer tissues were significantly higher than that in the adjacent normal tissues, while the expression level of miR-124-3p was significantly decreased in cancer tissues.CONCLUSIONSmiR-124-3p inhibits the migration and invasion of Gastric cancer by targeting ITGB3 in gastric cancer cells. Our results suggested that miR-124-3p and ITGB3 may reasonably serve as a promising therapeutic target. |
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| Keywords: | MicroRNA Integrin Gastric cancer Tumor migration and invasion Mechanism |
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