胶质瘤增殖活性和肿瘤基因蛋白表达及其意义

目的探讨胶质瘤增殖活性和肿瘤基因蛋白表达与分化和预后间的关系。方法应用免疫组化和图像分析技术对１２４例脑胶质瘤增殖细胞核抗原（ＰＣＮＡ）和几种肿瘤基因蛋白进行定性、定量研究。结果ＰＣＮＡ反应强度与级别和预后显著相关;ｃ－ｅｒｂＢ－２蛋白表达在分化程度高的胶质瘤强于分化程度低者,存活５年以上的病例阳性强于存活５年以下者。ＥＧＦ（４０．０％）、ＥＧＦＲ（９１．４％）和ｐ２１ｒａｓ（５３．３％）与分级和预后无显著关系。３种ｐ５３蛋白抗体阳性率均以Ⅱ～Ⅳ级胶质瘤为高;ｐ５３与ＰＣＮＡ反应强度平行。结论ｐ２１ｒａｓ、ｃ－ｅｒｂＢ－２、ＥＧＦ和ＥＧＦＲ改变在胶质瘤发生、发展过程中可能是早期事件,恶变后不再随级别增加而加重,而ｐ５３基因突变涉及胶质瘤发展各个阶段;ＰＣＮＡ能较好地反映胶质瘤的恶性程度。

Proliferative activities, oncoprotein expression and their significance in human gliomas

OBJECTIVE: To explore the relationship between the proliferative activities, oncoprotein expression, cell differentiation and prognosis of gliomas. METHODS: Immunohistochemistry and image analysis were used to study the expression of proliferating cell nuclear antigen (PCNA) and several oncoproteins both qualitatively and quantitatively in 124 brain gliomas. RESULTS: It was found that the intensities of PCNA reaction were significantly related to both the grade and prognosis of gliomas. Overexpression of c-erbB-2 protein was slightly stronger in well than in poorly-differenciated gliomas. Moreover, the reactions in patients who survived over 5 years were stronger than in those under 5 years. EGF (40.0%), EGFR (91.4%) and p21ras (53.3%) expression levels were related to neigher the grading nor prognosis of this tumor. The positive ratios of the three antibodies to p53 protein were higher in grades II-IV than in grade I gliomas. The intensity of p53 reaction was correlated to that of PCNA. CONCLUSION: It is suggested that the aberration of c-erbB-2, p21ras, EGF and EGFR might be the early events in the initiation and progression of gliomas, whereas p53 is involved in all stages of these tumors. PCNA could reflect the degree of malignancy to a certain extent.