| 用负向选择增富法筛选人GM-CSF cDNA |
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| 引用本文： | 孙英,马大龙,劳者歌,庞建,宋泉声,尉达民,税朝祥,陈萍生,王良绪,杨景山,唐岩. 用负向选择增富法筛选人GM-CSF cDNA[J]. 中国免疫学杂志, 1990, 0(4) |
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| 作者姓名： | 孙英 马大龙 劳者歌 庞建 宋泉声 尉达民 税朝祥 陈萍生 王良绪 杨景山 唐岩 |
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| 作者单位： | 北京医科大学免疫学教研室，北京医科大学免疫学教研室，北京医科大学免疫学教研室，北京医科大学免疫学教研室，北京医科大学免疫学教研室，北京医科大学第三医院血液研究室，北京医科大学第三医院血液研究室，北京医科大学第三医院血液研究室，北京医科大学第三医院血液研究室，北京医科大学组胚教研室，北京医科大学组胚教研室 北京 100083，北京 100083，北京 100083，北京 100083，北京 100083，北京 100083，北京 100083，北京 100083，北京 100083，北京 100083，北京 100083 |
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| 摘 要： | 用负向选择增富法成功地获得人GM-CSF cDNA克隆,筛选阳性率提高至1/170。用该cDNA转染的CDS-7细胞上清可测出集落刺激活性。经亚克隆化后在大肠杆菌表达出重组人GMCSF,可维持细胞株BMU-2的生长并促进急性非淋巴细胞白血病(ANLL)患者骨髓细胞的集落形成。
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| 关 键 词： | GM-csF cDNA 地高辛素探针 表达 |
Isolation, identification and expression of a cDNA clone encoding human GM-CSF |
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| Sun Ying,et al.. Isolation, identification and expression of a cDNA clone encoding human GM-CSF[J]. Chinese Journal of Immunology, 1990, 0(4) |
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| Authors: | Sun Ying et al. |
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| Affiliation: | Sun Ying,et al. Department of Immunology,Beijing Medical University,Beijing 100083,China |
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| Abstract: | One of the human GM-CSF cDNA was successfully isolated with a new screening method. Up 1/170 of positive rate was reached. The COS-7 cells were transfected with the cDNA clone and the resulting supernants contained colony-stimulatory activities. Human GM-CSF cDNA was also subcloned into a expression plasmid pKPL-2D and the unfused recombinant human GM-CSF was expressed in E. coli. The expressed products could maintain in vitro the growth of a cell line BMU- 2 and stimulate its colony formation. In addition, it could increase the colony formation of bone marrow cells from the patients with acute non-lymphatic leukemia (ANLL). |
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| Keywords: | GM-CSF cDNA Expression |
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