| 正反义细胞周期蛋白D1真核表达载体的构建与鉴定 |
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| 引用本文： | 闫克夏,刘秉慈,徐茗,尤宝荣,康宁.正反义细胞周期蛋白D1真核表达载体的构建与鉴定[J].卫生研究,2003,32(3):180-182. |
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| 作者姓名： | 闫克夏 刘秉慈 徐茗 尤宝荣 康宁 |
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| 作者单位： | 中国疾病预防控制中心职业卫生与中毒控制所,北京,100050 |
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| 基金项目： | 国家重点基础研究规划项目(2002 CB512906),国家自然科学基金资助项目(No.30028019) |
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| 摘 要： | 用基因导入和基因重组技术将外源基因细胞周期蛋白D1(cyclinD1)cDNA插入到真核表达载体pXJ41 neo上 ,并用电泳鉴定其结果。结果显示 :用HindIII酶切 ,出现 2 2 9bp、42 7bp及 75 5 4bp片段的重组体为正义pXJ41 cyclinD1真核表达载体 ;出现 42 7bp、1184bp及 65 99bp片段的重组体为反义pXJ41 cyclinD1真核表达载体 ,与理论分析结果相同。表明已成功重组和鉴定了正反义pXJ41 cyclinD1真核表达载体。为反义技术在环境及职业危害因素所致肿瘤研究中的应用提供有效的工具
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| 关 键 词： | 细胞周期 细胞周期蛋白D1 反义RNA 肿瘤 |
| 文章编号： | 1000-8020(2003)03-0180-03 |
| 修稿时间： | 2002年12月2日 |
Recombination and identification of sense and antisense cyclinD1 eukaryotic expression vectors |
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| Yan Kexia,Liu Bingci,Xu Ming,You Baorong,et al..Recombination and identification of sense and antisense cyclinD1 eukaryotic expression vectors[J].Journal of Hygiene Research,2003,32(3):180-182. |
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| Authors: | Yan Kexia Liu Bingci Xu Ming You Baorong |
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| Institution: | Institute of Occupational Health and Poisons Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. |
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| Abstract: | The exogenous gene cyclinD1 cDNA was inserted into eukaryotic expression vector pXJ41-neo by gene transduction and recombination techniques. The recombinant was detected by electrophoresis. Results showed that the recombinant sense plasmid (pXJ41-cyclinD1) was digested by HindIII into 229bp, 427bp and 7554bp; while the recombinant antisense plasmid was digested into 427bp, 1184bp and 6599bp, implying that sense and antisense pXJ41-cyclinD1 eukaryotic expression vectors were successfully recombined. The finding from this study offers an effective tool to reveal the mechanism of the carcinogenesis of environmental and occupational harzards. |
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| Keywords: | recombinant antisense cyclinD1 |
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